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Effects of non-esterified fatty acids on the gluconeogenesis in bovine hepatocytes

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Abstract

Dairy cows experience an increased demand for glucose to support milk production. However, negative energy balance is a common condition in peripartum cows. In response, fat mobilization provides non-esterified fatty acids (NEFAs) for oxidation in the liver to generate ATP. To investigate the effects of NEFAs on gluconeogenesis, the expression and enzyme activity of pyruvate carboxylase (PC) and phosphoenolpyruvate carboxylase (PEPCK) in cultured bovine hepatocytes were evaluated by quantitative polymerase chain reaction and spectrophotometry, respectively. The results showed that PC and PEPCK mRNA levels were marked decreased when the NEFAs concentrations exceeded 0.5 and 1.5 mmol/l, respectively. The PC and PEPCK enzyme activity showed significantly decreased when the NEFAs concentrations exceeded 1.5 and 0.5 mmol/l, respectively. These findings indicate that high circulating levels of NEFAs inhibit hepatocyte gluconeogenesis, thereby promoting negative energy balance.

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Acknowledgments

This study was supported by a grant from the National Key Basic Research Program of China (No. 2011CB100800).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, 130062, Jilin, China

    Xinwei Li, Xiaobing Li, Ge Bai, Hui Chen, Qinghua Deng, Zhaoxi Liu, Liang Zhang, Guowen Liu & Zhe Wang

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  1. Xinwei Li
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  2. Xiaobing Li
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  3. Ge Bai
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  4. Hui Chen
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  5. Qinghua Deng
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  6. Zhaoxi Liu
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  7. Liang Zhang
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  8. Guowen Liu
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  9. Zhe Wang
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Corresponding authors

Correspondence to Guowen Liu or Zhe Wang.

Additional information

Xinwei Li, Xiaobing Li, Ge Bai, and Hui Chen contributed equally to this study.

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Li, X., Li, X., Bai, G. et al. Effects of non-esterified fatty acids on the gluconeogenesis in bovine hepatocytes. Mol Cell Biochem 359, 385–388 (2012). https://doi.org/10.1007/s11010-011-1032-x

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  • DOI: https://doi.org/10.1007/s11010-011-1032-x

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