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Down-regulation of CK2 activity results in a decrease in the level of cdc25C phosphatase in different prostate cancer cell lines

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Abstract

Protein kinase CK2 is implicated in the regulation of the cell cycle. In addition to a variety of functions, CK2 has anti-apoptotic properties. So far the role of CK2 linking both pathways in the cell is not clear. Some years ago we found that CK2 phosphorylates cdc25C, one member of the cdc25 family of proteins. In this study, we showed that inhibition of CK2 activity by three different inhibitors led to a down-regulation of the level of cdc25C. Inhibition of CK2 activity by transfecting the dominant-negative CK2α subunit also resulted in a down-regulation of the level of cdc25C whereas inhibition of CK2α′ had no effect on the cdc25C level. In both cases, we observed apoptosis by PARP cleavage as well as by an increase in γH2AX phosphorylation. These results show that down-regulation of the level of cdc25C is not a prerequisite for the induction of apoptosis.

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Acknowledgments

We thank D. Litchfield, Ontario, for the gift of the original vectors with the CK2 dominant-negative mutants. We also thank Nathaniel Saidu for editing the manuscript.

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Author notes

  1. Andrea Hessenauer

    Present address: Allgemeine Zoologie und Endokrinologie, Universität Ulm, M 24, 89069, Ulm, Germany

Authors and Affiliations

  1. Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Gebäude 44, 66421, Homburg, Germany

    Carolin C. Schneider, Claudia Götz, Andrea Hessenauer, Jürgen Günther, Sabine Kartarius & Mathias Montenarh

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  1. Carolin C. Schneider
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  2. Claudia Götz
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  3. Andrea Hessenauer
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  4. Jürgen Günther
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  5. Sabine Kartarius
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  6. Mathias Montenarh
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Corresponding author

Correspondence to Mathias Montenarh.

Additional information

Carolin C. Schneider and Claudia Götz contributed equally to this study.

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Schneider, C.C., Götz, C., Hessenauer, A. et al. Down-regulation of CK2 activity results in a decrease in the level of cdc25C phosphatase in different prostate cancer cell lines. Mol Cell Biochem 356, 177–184 (2011). https://doi.org/10.1007/s11010-011-0946-7

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  • DOI: https://doi.org/10.1007/s11010-011-0946-7

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