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Human amniotic membrane-derived mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles: the effect on neuron-like differentiation in vitro

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Abstract

Mesenchymal stem cells (MSCs) have the potential for self-renewal and multipotential differentiation to regenerate damaged tissues or recover functional absence in diseases. Superparamagnetic iron oxide nanoparticles (SPIONs) are used as contrast agents in magnetic resonance imaging (MRI) for labeling cells in vitro and for tracking SPION-labeled cells after transplantation in vivo. Human amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) have the capacity for neuron-like differentiation that could be used to cure central nervous system (CNS) diseases. The study investigated the impacts of cytotoxicity of SPIONs on neuron-like differentiation of hAM-dMSCs in both single (1×) and multiple (4×) SPIONs-labeled methods. hAM-dMSCs could be efficiently labeled at safe concentrations of SPIONs (≤14 μg/ml) without significantly affecting their viability (>80% after a MTT assay), special surface antigens (CD29, CD44, CD90, CD105 through flow cytometry), and neuron-like differentiation (nestin and neuron-specific enolase through immunocytochemistry and reverse transcription polymerase chain reaction). Compared with multiple (4×) SPION-labeled methods, a single (1×) SPION-labeled method avoided multiple SPION-labeled hAM-dMSCs and minimized the impact of cytotoxicity of SPIONs on neuron-like differentiation of hAM-dMSCs. Under safe concentrations of SPIONs, a single (1×) SPION-labeled method provided appropriate viability for SPIONs-labeled hAM-dMSCs and facilitated the MRI evaluation of hAM-dMSCs after transplantation.

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Abbreviations

MSCs:

Mesenchymal stem cells

SPIONs:

Super paramagnetic iron oxide nanoparticles

hAM-dMSCs:

Human amniotic membrane-derived mesenchymal stem cells

MRI:

Magnetic resonance imaging

CNS:

Central nervous system

DMEM-LG:

Dulbecco’s modified eagle’s medium–low glucose

bFGF:

Basic fibroblast growth factor

NGF:

Nerve growth factor

RA:

Retinoic acid

CGM:

Cytokine growth medium

CIM:

Cytokine induction medium

RT-PCR:

Reverse transcription polymerase chain reaction

MTT:

3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide

NSE:

Neuron-specific enolase

GFAP:

Glia fibrillary acid protein

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Acknowledgments

This research was supported by Henan Medical Science and Technology Research Projects of Health Department of Henan Province, People’s Republic of China (No. 200702003); Henan Medical Technological Innovation Projects of Health Department of Henan Province, People’s Republic of China (No. 2005018).

Conflict of interest

The authors state no conflicts of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, First Hospital of Zhengzhou University, Zhengzhou, 450052, Henan Province, People’s Republic of China

    Guangwei Zeng, Keliang Chang, Hongliang Jiao & Bo Yang

  2. Department of Plastic, Reconstructive and Burns Surgery, First Hospital of Henan University of Science and Technology, LuoYang, 471003, Henan Province, People’s Republic of China

    Guangwei Zeng

  3. Department of Onchology, First Hospital of Henan University of Science and Technology, LuoYang, 471003, Henan Province, People’s Republic of China

    Gongping Wang

  4. Department of Bioengineering, Zhengzhou University, Zhengzhou, 450001, Henan Province, People’s Republic of China

    Fangxia Guan

  5. Department of Radiology, First Hospital of Henan University of Science and Technology, LuoYang, 471003, Henan Province, People’s Republic of China

    Wanqin Gao

  6. Department of Pharmacology, Medical School of Henan University of Science and Technology, LuoYang, 471003, Henan Province, People’s Republic of China

    Shoumin Xi

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  1. Guangwei Zeng
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  8. Bo Yang
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Correspondence to Bo Yang.

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Zeng, G., Wang, G., Guan, F. et al. Human amniotic membrane-derived mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles: the effect on neuron-like differentiation in vitro. Mol Cell Biochem 357, 331–341 (2011). https://doi.org/10.1007/s11010-011-0904-4

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  • DOI: https://doi.org/10.1007/s11010-011-0904-4

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