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Effects of stable knockdown of Aurora kinase A on proliferation, migration, chromosomal instability, and expression of focal adhesion kinase and matrix metalloproteinase-2 in HEp-2 cells

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Abstract

Overexpression of Aurora kinase A (AURKA) is frequently observed in various cancers, including laryngeal squamous cell carcinoma (LSCC). We investigated the effects of knockdown of AURKA on laryngeal cancer HEp-2 cells both in vitro and in vivo. A plasmid containing short hairpin (sh)RNA against AURKA was constructed and transfected into HEp-2. Measurements included the CCK-8 assay for viability and proliferation, flow cytometry for apoptosis and effects on the mitotic checkpoint, a trans-well assay for migration, immunofluorescence for assessment of genomic instability, and western blotting for protein expression. AURKA knockdown inhibited proliferation, migration, and colony formation in vitro and tumorigenicity in vivo. The knockdown induced the accumulation of cells in G2-M phase and eventual apoptosis. Knockdown of AURKA caused delayed entry into mitosis after treatment with nocodazole, reduced chromosomal instability, and decreased expression of focal adhesion kinase (FAK), phosphorylated FAK, and matrix metalloproteinase-2 (MMP-2), key regulators in cell adhesion and invasion. Knockdown of AURKA inhibits the growth and invasiveness of this LSCC cell line both in vitro and in vivo. These effects may partially result from the reduced expression of FAK and MMP-2. Knockdown of AURKA expression may represent a promising therapeutic strategy for the treatment of LSCC.

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Acknowledgments

This study was supported by the Shanghai Science and Technology Committee (09411950600), the Chinese National Natural Science Foundation (30670939).

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Otolaryngology, Head and Neck Surgery, Eye Ear Nose and Throat Hospital, Fudan University, 83 FenYang Road, Shanghai, 200031, China

    Hao Zhang & Liang Zhou

  2. Department of Surgery, Ruijin Hospital, School of Medicine, Jiao Tong University, 197 second Ruijin Road, Shanghai, 200025, China

    Xuehua Chen & Bingya Liu

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  1. Hao Zhang
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  4. Liang Zhou
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Correspondence to Liang Zhou.

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Zhang, H., Chen, X., Liu, B. et al. Effects of stable knockdown of Aurora kinase A on proliferation, migration, chromosomal instability, and expression of focal adhesion kinase and matrix metalloproteinase-2 in HEp-2 cells. Mol Cell Biochem 357, 95–106 (2011). https://doi.org/10.1007/s11010-011-0879-1

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  • DOI: https://doi.org/10.1007/s11010-011-0879-1

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