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Mouse transmembrane BAX inhibitor Motif 3 (Tmbim3) encodes a 38 kDa transmembrane protein expressed in the central nervous system

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Abstract

Cell survival proteins play an important role throughout nervous system development, normal physiological processes, and pathological conditions. Transmembrane BAX inhibitor motif 3 (TMBIM3, also known as GRINA), is a member of a family of proteins that contain a conserved BAX inhibitor-1 motif. This family of proteins includes several members that have been shown to protect cells from apoptosis. In this study, the authors show that TMBIM3 is expressed in the brain including high levels in the hippocampus. Biochemical and sequence analysis of TMBIM3 demonstrates that the rat, murine, and human genes encode an approximately 38 kDa protein with a predicted seven transmembrane domain topology. A Tmbim3 knockout mouse line did not have an obvious phenotype, but may prove useful in future studies of this family of proteins.

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Acknowledgments

The authors thank Dr. Chang-Yu Wang for the whole brain membrane fraction. The authors thank Dr. Dragan Maric for his FACS expertise and analysis of the Tmbim3 cell survival experiments, and Dr. Jim Pickle for assistance with floxing. The authors thank Dr. Sara Szuchet for initiating this project. This research was supported by the Intramural Research Program of the National Institute of Neurological Disorders and Stroke (NINDS).

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Authors and Affiliations

  1. Section of Developmental Genetics, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA

    Joseph A. Nielsen, Meredith A. Chambers, Elena Romm, Laurel Yong-Hwa Lee, Jo Ann Berndt & Lynn D. Hudson

  2. Center for Devices and Radiological Health, Food and Drug Administration, WO66 Room G431, 10903 New Hampshire Avenue, Silver Spring, MD, 20993, USA

    Joseph A. Nielsen

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  1. Joseph A. Nielsen
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  2. Meredith A. Chambers
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  3. Elena Romm
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  4. Laurel Yong-Hwa Lee
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  5. Jo Ann Berndt
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  6. Lynn D. Hudson
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Correspondence to Joseph A. Nielsen.

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Nielsen, J.A., Chambers, M.A., Romm, E. et al. Mouse transmembrane BAX inhibitor Motif 3 (Tmbim3) encodes a 38 kDa transmembrane protein expressed in the central nervous system. Mol Cell Biochem 357, 73–81 (2011). https://doi.org/10.1007/s11010-011-0877-3

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  • DOI: https://doi.org/10.1007/s11010-011-0877-3

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