Oxidative stress is triggered by the wound which results in the production of reactive oxygen species (ROS), thereby delaying normal wound repair. Therefore, it is important to reduce the level of ROS to improve healing. A known antioxidant, dehydrozingerone (DHZ) was synthesized and selected for the study. The authors aimed to investigate the wound healing action of topical (100 mg/wound) and systemic (100 mg/kg, p. o.). DHZ on different wound models in normal and dexamethasone (DEX)-suppressed healing. Topical DHZ showed a significant (P < 0.05) rise in tensile strength when compared to control in normal healing. Significant (P < 0.05) wound closure was observed from 3 to 9 days in DHZ oral and gel treated groups. There was a significant (P < 0.05) rise in hydroxyproline content with the DHZ treated groups when compared to control. Systemic DHZ exhibited a significant (P < 0.05) increase in lysyl oxidase (LO) levels of 3.73 ± 0.15 nmol of H2O2 when compared to control. In DEX-suppressed healing, showed good pro-healing activity with respect to the parameters mentioned above. DHZ treatment exhibited a parabolic dose response of ROS inhibition with a plateau effect at 75 μM. There was a steady and constant increase in the % NO inhibition with increasing doses of DHZ. Oral DHZ is effective in accelerating the healing process in both normal and dexamethasone-suppressed wounds. Our study suggests that DHZ (half analog of curcumin) supplementation reduces the steroid-induced delay in wound healing.
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Authors are thankful to Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University for providing the facilities to carry out the project. The authors would also like to acknowledge the financial support offered by the All India Council of Technical Education (AICTE), New Delhi through RPS (Research Promotion Scheme) in purchase of the Biotek luminometer and microplate reader used in this study.
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Rao, M.C., Sudheendra, A.T., Nayak, P.G. et al. Effect of Dehydrozingerone, a half analog of curcumin on dexamethasone-delayed wound healing in albino rats. Mol Cell Biochem 355, 249 (2011). https://doi.org/10.1007/s11010-011-0861-y
- Lysyl oxidase
- Superoxide dismutase