Induction of pluripotent stem cells transplantation therapy for ischemic stroke

Abstract

Stroke can cause permanent neurological damage, complications, and even death. However, there is no treatment exists to restore its lost function. Human embryonic stems transplantation therapy was a novel and potential therapeutic approach for stroke. However, as we have seen, the ethical controversy pertains to embryonic stem cell research. Human induced pluripotent stem cells (iPSCs) are the latest generation of stem cells that may be a solution to the controversy of using embryonic cells. In our study, we generated iPSCs from adult human fibroblasts by introduction of four defined transcription factors (Oct4, Sox2, Nanog, and Lin-28). And then, we investigated the efficacy of iPSCs transplantation therapy for stroke on the animal models of middle cerebral artery occlusion. Surprisingly, we found that transplanted iPSCs migrated to injured brain areas, and differentiated into neuron-like cells successfully. After 4–16 days iPSCs grafting, sensorimotor function of rats has been improved significantly. In one word, we may prove that iPSCs therapy in stroke to be an effective form of treatment.

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Abbreviations

CNS:

Central nervous system

ES:

Embryonic stem

%HLV:

Percentage hemisphere lesion volume

HESC:

Human embryonic stem cells

iPSCs:

Induced pluripotent stem cells

MCAO:

Middle cerebral artery occlusion

SCNT:

Somatic cells nuclear transfer

TTC:

2,3,5-Triphenyltetrazolium chloride

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Acknowledgments

The authors would like to thank members of Professor Wu Xinzhong’s laboratory for their helpful discussion. This work was partially supported by Natural Science Foundation of China, grant number, 30570632. Pudong New Area, the leading health systems, medical personnel training programs, grant number, PWRq2007-03.

Conflict of interest

All authors have no competing interest to declare.

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Correspondence to Jian Sun or Qiang Dong.

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Mei Jiang and Lei Lv have contributed equally to this work.

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Jiang, M., Lv, L., Ji, H. et al. Induction of pluripotent stem cells transplantation therapy for ischemic stroke. Mol Cell Biochem 354, 67–75 (2011). https://doi.org/10.1007/s11010-011-0806-5

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Keywords

  • Brain ischemia
  • Induced pluripotent stem cells
  • Fibroblasts
  • Transcription factors
  • Transplantation therapy