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Role of cardiac isoform of alpha-2 macroglobulin in diabetic myocardium

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Abstract

Earlier studies from one of the investigator’s laboratory have demonstrated the presence of a high molecular weight protein (182 kDa) in the blood serum of laboratory animals subjected to pressure-induced cardiac hypertrophy and suggested that this protein may be involved in the development of cardiac hypertrophy. Studies have shown that this protein is also involved in earlier stages of cardiac complications associated with diabetes, but the role of this protein in diabetic heart is less understood. So we aimed to check whether this protein is having any protective role in diabetic heart. The protein was purified from serum of rats induced with cardiac hypertrophy and the purified protein was injected through tail vein of diabetic rats for further studies. The results of various antioxidant enzymes and the TBARS levels have indicated the antioxidant activity of this protein. Real-time PCR analysis of gene expression revealed the upregulation of certain muscle-specific genes like β-MHC, MLC-2, and skeletal α actin in diabetic group and also in presence of 182-kDa protein. The results further showed a down regulation of genes such as cardiac α-actin and α- MHC implicating the role of this protein in the development of cardiac hypertrophy in diabetes. Increased cardiac hypertrophy as revealed by the expression of various genes and improved antioxidant potential in presence of 182 kDa protein in diabetes at the earlier stages is beneficial for counteracting the myocardial damage associated with diabetes.

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Acknowledgments

This study was supported by Kerala State Council for Science Technology and Environment, Sasthra Bhavan, Pattom, Kerala, We thank Dr. P. R Sudhakaran, former Head, Department of Biochemistry, University of Kerala, Kerala for his kindly support during the course of the study. We also thank Dr. Akhilesh Pandey to have allowed the use of proteomics facility at Institute of Bioinformatics, Bangalore.

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  1. Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, 695581, India

    Sowmya Soman, C. S. Manju, Arun A. Rauf, M. Indira & C. Rajamanickam

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  1. Sowmya Soman
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  2. C. S. Manju
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  3. Arun A. Rauf
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  4. M. Indira
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  5. C. Rajamanickam
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Correspondence to C. Rajamanickam.

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Soman, S., Manju, C.S., Rauf, A.A. et al. Role of cardiac isoform of alpha-2 macroglobulin in diabetic myocardium. Mol Cell Biochem 350, 229–235 (2011). https://doi.org/10.1007/s11010-010-0702-4

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  • DOI: https://doi.org/10.1007/s11010-010-0702-4

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