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Association of aorta intima permeability with myosin light chain kinase expression in hypercholesterolemic rabbits

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Abstract

The development of hypercholesterolemia is a multifactorial process in which elevated plasma cholesterol levels play a central role. This study analyzed the variability of the expression and activity of myosin light chain kinase (MLCK) and endothelial permeability in the artery wall of rabbits after feeding the animals with a normal or a high-cholesterol diet. Hypercholesterolemia was induced by a high-cholesterol diet for 4 weeks. Aortas were removed and analyzed for endothelial permeability and MLCK expression. Samples of the arterial media were analyzed for MLCK activity and expression. A selective MLCK inhibitor 1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride (ML7) were used in hypercholesterolemia rabbit (1 mg/kg body weight). The aortas of high-cholesterol diet rabbits showed an increase in MLCK expression and activity (nearly threefold compare with control) as well as endothelial permeability. ML7 inhibit MLC phosphorylation and MLCK activity (nearly twofold compare with control) and endothelial permeability stimulated by cholesterol. These results indicate for the first time that hypercholesterolemia may be associated with MLCK expression and activity through which endothelial permeability is increased.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (No. 30971226 and 81070232) and Natural Science Foundation of Anhui province of China (No. 090413116).

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Authors and Affiliations

  1. Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei, 230032, Anhui Province, People’s Republic of China

    Hua-Qing Zhu, Qing Zhou, Zhi-Kui Jiang & Yuan Wang

  2. Department of Respiratory Disease, Anhui Medical University, Hefei, 230032, Anhui, People’s Republic of China

    Shu-Yu Gui

  3. Anhui Province Key Laboratory of Genomic Research and the Key Laboratory of Gene Resource Utilization for Severe Disease, Ministry of Education, Anhui Medical University, Hefei, 230032, Anhui, People’s Republic of China

    Hua-Qing Zhu, Qing Zhou, Zhi-Kui Jiang, Shu-Yu Gui & Yuan Wang

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  1. Hua-Qing Zhu
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  2. Qing Zhou
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  4. Shu-Yu Gui
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  5. Yuan Wang
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Correspondence to Yuan Wang.

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Hua-Qing Zhu and Qing Zhou contributed equally to this work.

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Zhu, HQ., Zhou, Q., Jiang, ZK. et al. Association of aorta intima permeability with myosin light chain kinase expression in hypercholesterolemic rabbits. Mol Cell Biochem 347, 209–215 (2011). https://doi.org/10.1007/s11010-010-0630-3

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  • DOI: https://doi.org/10.1007/s11010-010-0630-3

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