Abstract
We used forebrain-specific conditional presenilin 1 (PS1) and presenilin 2 (PS2) double knockout mice (dKO mice), which exhibit neurodegenerative disease-like symptoms, including inflammation of the brain and periphery, to investigate whether periodontal and salivary tissues display alterations. Mandibles were dissected for alveolar bone height analysis. Maxillae were fixed and decalcified for histological observation and osteoclast detection. Submandibular glands were fixed for histological observation. The submandibular gland and the gingiva of the mandibular incisor teeth were used to assay inflammatory mediators. At 9 months, the number of osteoclasts had significantly increased in the periodontal ligament and the periodontal tissues exhibited obvious histomorphological abnormalities in the dKO mice compared to the control mice at the same age. Alveolar bone loss in dKO mice increased with age. The salivary tissues in dKO mice exhibited obvious age-dependent histomorphological abnormalities. The levels of the inflammatory mediators IL-1β, TNF-α, and GM-CSF in the submandibular gland and gingiva also increased in an age-dependent manner. These findings suggest that inflammation in the dKO brain could expand to the periphery, including the oral tissue, which could ultimately induce abnormalities in the periodontal and salivary tissues.
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Acknowledgments
We deeply appreciate the generosity of Dr. Joe Tsien (Medical College of Georgia, USA) in providing the PS1/PS2 double knockout mice. This study was supported by grants from National Natural Science Foundation of China (30840031, 30870790, 30970726, 81070876) and from Shanghai (0852NM03600, 10JC1411200, 11ZZ38), and was supported, in part, by the 973 Project (2009CB918402) and the Fundamental Research Funds for the Central Universities.
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Han, W., Ji, T., Wang, L. et al. Abnormalities in periodontal and salivary tissues in conditional presenilin 1 and presenilin 2 double knockout mice. Mol Cell Biochem 347, 13–20 (2011). https://doi.org/10.1007/s11010-010-0607-2
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DOI: https://doi.org/10.1007/s11010-010-0607-2