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Regulation of adhesion by vascular endothelial growth factor in HaCaT cells

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Abstract

Cell adhesion is an important process during morphogenesis, differentiation, and homeostasis in cell biology. The role of vascular endothelial growth factor (VEGF) in cell adhesion of keratinocytes is unclear. In our study, a human keratinocyte cell line, HaCaT cells, which mimics various properties of normal epidermal keratinocytes, was included to elucidate the effect of VEGF on cell–cell adhesion and cell–plate adhesion. Expression of adhesion molecules account for cell adhesion and signal transduction pathways involved in the effect of VEGF on adhesion of HaCaT cells were further investigated. Significant increase of cell–cell adhesion but decrease of the cell–plate adhesion of HaCaT cells induced by VEGF165 was detected. VEGF increases expression of E-cadherin, but inhibits expression of integrin α6β4 subunit. VEGF165 at 100 ng/ml activates extracellular signal-regulated kinase. These changes of cell adhesion induced by VEGF were blocked by ERK and VEGFR-2 inhibitor. Our findings suggest that VEGF may modulate cell adhesion of HaCaT cells partly through activation of VEGFR-2/ERK1/2 signaling pathways.

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Acknowledgment

This study was supported by Grants from the National Natural Science Foundation of China (NSFC) (no. 30771945) and the Natural Science Foundation of Zhejiang Province (no. 399126).

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Authors and Affiliations

  1. Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, 310009, China

    ChunMing Li, XiaoYong Man, Wei Li, Jiong Zhou, JiaQi Chen, SuiQing Cai & Min Zheng

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  1. ChunMing Li
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  2. XiaoYong Man
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  3. Wei Li
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  7. Min Zheng
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Correspondence to Min Zheng.

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Li, C., Man, X., Li, W. et al. Regulation of adhesion by vascular endothelial growth factor in HaCaT cells. Mol Cell Biochem 346, 173–178 (2011). https://doi.org/10.1007/s11010-010-0602-7

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  • DOI: https://doi.org/10.1007/s11010-010-0602-7

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