Abstract
Integrin beta 4 (ITGB4) is a structural adhesion molecule which engages in maintaining the integrity of airway epithelial cells. Its specific cytomembrane structural feature strongly indicates that ITGB4 may engage in many signaling pathways and physiologic processes. However, in addition to adhesion, the specific biologic significance of ITGB4 in airway epithelial cells is almost unknown. In this article, we investigated the expression and functional properties of ITGB4 in airway epithelial cells in vivo and in vitro. Human bronchial epithelial cell line (16HBE14O-cells) and primary rat tracheal epithelial cells (RTE cells) were used to determine ITGB4 expression under ozone tress or mechanical damage, respectively. An ovalbumin (OVA)-challenged asthma model was used to investigate ITGB4 expression after antigen exposure in vivo. In addition, an ITGB4 overexpression vector and ITGB4 silence virus vector were constructed and transfected into RTE cells. Then, wound repair ability and anti-oxidation capacity was evaluated. Our results demonstrated that, on the edge of mechanically wounded cell areas, ITGB4 expression was increased after mechanical injury. After ozone stress, upregulation expression of ITGB4 was also detected. In the OVA-challenged asthma model, ITGB4 expression was decreased on airway epithelial cells accompanying with structural disruption and damage of anti-oxidation capacity. Besides, our study revealed that upregulation of ITGB4 promotes wound repair ability and anti-oxidative ability, while such abilities were blocked when ITGB4 was silenced. Taken together, these results showed that ITGB4 was a new interesting molecule involved in the regulation of wound repair and anti-oxidation processes for airway epithelial cells.
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Acknowledgments
The authors sincerely appreciate the editor and reviewers for their positive and insightful comments. We thank Professor Gruenert from the San Francisco Branch Campus of the University of California for the gift of the immortalized human bronchial epithelial cell line, 16HBE14O. This study was supported by grant #30800504 from the National Natural Science Foundation of China, the key projects #2007FJ3009 from Hunan Science and Technology plans, and the construct program of the key discipline in Hunan province.
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Liu, C., Liu, Hj., Xiang, Y. et al. Wound repair and anti-oxidative capacity is regulated by ITGB4 in airway epithelial cells. Mol Cell Biochem 341, 259–269 (2010). https://doi.org/10.1007/s11010-010-0457-y
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DOI: https://doi.org/10.1007/s11010-010-0457-y