Abstract
Lysosomes are a cell organelle type processing antimicrobial activity. Here, we investigate the lysosomal activity in a primary cell line, bovine aortic endothelial cells (BAECs), during cellular aging, based on the antimicrobial activity of lysosomes isolated from BAECs at cell passages 4, 6, 8, and 10. Cytochemical analysis of lysosomes with LysoTracker reagent revealed the number of lysosome-like organelles surrounding the nucleus initially increased drastically in the BAECs and continued increasing gradually until passage 10. The lysosomes isolated from each successive passage of BAECs exhibited increased antimicrobial activity against Escherichia coli, and, in addition, an age-dependent increase in lysosome intensity coincided with increased lysosomal antimicrobial activity.
This is a preview of subscription content, log in via an institution to check access.
References
Dice JF (2002) Lysosomal pathways protein degradation. Landes Biocience, Austin, TX
Li SC, Kane PM (2009) The yeast lysosome-like vacuole: endpoint and crossroads. Biochim Biophys Acta 1793:650–663
Cuervo AM, Dice JF (2000) When lysosomes get old. Exp Gerontol 35:19–131
Brank U, Ericsson JL, Pontén J, Wertermark B (1973) Residual bodies and “aging” in cultured human glia cells. Effect of entrance into phase 3 and prolonged period of confluence. Exp Eye Res 79:1–14
Robbins E, Levine EM, Eagle H (1970) Morphologic changes accompanying senescence of cultured human diploid cells. J Exp Med 131:1211–1222
Chondrogianni N, Fragoulis EG, Gonos ES (2002) Protein degradation during aging: the lysosome-, the captain- and the proteasome-dependent cellular proteolytic systems. Biogerontology 3:121–123
Homma Y, Tsunoda M, Kasai H (1994) Evidence for the accumulation of oxidative stress during cellular aging of human diploid fibroblasts. Biochem Biophys Res Commun 203:1063–1068
June T, Höhn A, Catalgol B, Grune T (2009) Age-related differences in oxidative protein-damage in young and senescent fibroblasts. Arch Biochem Biophys 483:127–135
Kurz T, Terman A, Gustafsson B, Brunk UT (2008) Lysosomes and oxidative stress in aging and apoptosis. Biochim Biophys Acta 1780:1291–1303
Zhao M, Antunes F, Eaton JW, Brunk UT (2003) Lysosomal enzymes promote mitochondrial oxidant production, cytochrome C release and apoptosis. Eur J Biochem 270:3778–3786
Yoon J, Park J-M, Jung S-K, Kim K-J, Kim Y-H, Min J (2009) Characterization of antimicrobial activity of the lysosomes isolated from Saccharomyces cerevisiae. Curr Microbiol 59:48–52
Chiaraviglio L, Brown DA, Kirby JE (2008) Infection of cultured human endothelial cells by Legionella pneumophila. PLoS ONE 3:1–5
Trifillis AL, Craggs MH (1986) Effects of cyclosporine on human endothelial cell cultures. Toxicol Pathol 14:210–212
Kim HP, Lee JY, Jeong JK, Bae SW, Lee HK, Jo I (1999) Nongenomic stimulation of nitric oxide release by estrogen is mediated by estrogen receptor α localized in caveolae. Biochem Biophys Res Commun 263:257–262
Yoon J, Park J-M, Kim K-J, Kim Y-H, Min J (2009) Antimicrobial activity of the cell organelle, lysosomes isolated from egg white. J Microbiol Biotechnol 19:1364–1368
Illinger D, Kuhry JG (1994) The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol 125:783–794
Acknowledgments
This study was supported by a 2008 research grant from Chungbuk National University. The authors are grateful for these supports.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Yoon, J., Kim, KJ., Choi, YW. et al. The dependence of enhanced lysosomal activity on the cellular aging of bovine aortic endothelial cells. Mol Cell Biochem 340, 175–178 (2010). https://doi.org/10.1007/s11010-010-0415-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11010-010-0415-8