Abstract
NYGGF4 is a recently discovered gene that is involved in obesity-associated insulin resistance. The exact mechanism by which NYGGF4 induces insulin resistance has not yet been fully elucidated. In this study, we demonstrated that the overexpression of NYGGF4 in 3T3-L1 adipocytes decreased mitochondrial mass, mitochondrial DNA, and intracellular ATP synthesis. In addition, NYGGF4 overexpression also led to an imbalance of the mitochondrial dynamics and excess intracellular ROS production. Collectively, our results indicated that the overexpression of NYGGF4 caused mitochondrial dysfunction in adipocytes, which might be responsible for the development of NYGGF4-induced insulin resistance.
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Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (Grant Number: 30571978, 30973231) and Program of Medical Leading Talent in Jiangsu Province (Grant Number: LJ200624)
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YaPing Zhao and Chunmei Zhang contributed equally to this work.
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Zhao, Y., Zhang, C., Chen, X. et al. Overexpression of NYGGF4 (PID1) induces mitochondrial impairment in 3T3-L1 adipocytes. Mol Cell Biochem 340, 41–48 (2010). https://doi.org/10.1007/s11010-010-0398-5
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DOI: https://doi.org/10.1007/s11010-010-0398-5