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Analysis of GSTM1 and GSTT1 polymorphisms in circulating plasma DNA of lung cancer patients

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Abstract

The concentration of free circulating plasma DNA and the genetic profile of patients suffering from various types of tumors were studied in an effort to increase the understanding of the biomarkers and genetic factors involved in predisposing an individual to lung cancer (LC). The polymorphic inheritance of glutathione S-transferases (GST), which modulate the effects of various genotoxic agents, especially those derived from benzo[a]pyrene, one of the main tobacco carcinogens, has been implicated in both cancer risk and prognostics. We investigated gene polymorphisms in the blood serum of patients previously diagnosed at the Pneumology Division of the Clementino Fraga Filho University Hospital of the Federal University of Rio de Janeiro and in buccal swab samples of exfoliated oral cells obtained from a population of healthy controls. The distribution of GSTM1 and GSTT1 polymorphisms was not significantly different between LC patients and the controls, suggesting that GSTM1 and GSTT1 alone or in combination are not independent risk factors for LC. However, a close relationship between smoking status and LC was clearly demonstrated. The most significant risk for LC concerning tobacco smoking was found in the association of null genotypes for GSTM1 and GSTT1 (P < 0.0001).

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Acknowledgements

We thank Dr Marcos Paschoal from the Pneumology Division of the Clementino Fraga Filho University Hospital of the Federal University of Rio de Janeiro for kindly providing clinical information of the patients and Dr Marcia Tie Kawamura for helping with plasma/serum collection and preparation. We also acknowledge Fernanda Ribeiro and Brenda Maiolino for providing technical support and generously donating DNA samples of the controls as well as Judy Grevan for editing the manuscript. This work was supported by FAPERJ (Rio de Janeiro, RJ, Brazil), CNPq (Brasilia, DF, Brazil) and the Fundação do Cancer/Oncobiologia.

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Authors and Affiliations

  1. IBCCF, Universidade Federal do Rio de Janeiro-UFRJ, Av Carlos Chagas Filho, Ilha do Fundão CCS-Bloco G, CEP 21949-900, Rio de Janeiro, RJ, Brazil

    Rosa Estela Caseira Cabral, Januario Bispo Cabral-Neto & Maria da Gloria Costa Carvalho

  2. Departamento de Biofísica e Biometria, IBRAG, UERJ, Rio de Janeiro, RJ, Brazil

    Adriano Caldeira-de-Araujo

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  1. Rosa Estela Caseira Cabral
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Correspondence to Rosa Estela Caseira Cabral.

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Cabral, R.E.C., Caldeira-de-Araujo, A., Cabral-Neto, J.B. et al. Analysis of GSTM1 and GSTT1 polymorphisms in circulating plasma DNA of lung cancer patients. Mol Cell Biochem 338, 263–269 (2010). https://doi.org/10.1007/s11010-009-0360-6

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  • DOI: https://doi.org/10.1007/s11010-009-0360-6

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