Skip to main content
SpringerLink
Log in

The interaction between interferon-induced protein with tetratricopeptide repeats-1 and eukaryotic elongation factor-1A

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

It has been shown previously that in mammalian cells, interferon-induced protein with tetratricopeptide repeats-1(IFIT1) is rapidly synthesized in response to viral infection, functions as an inhibitor of translation by binding to the eukaryotic initiation factor-3, and consequently assigns resistive activity against viral invasion to cells. It has also been reported that IFIT1 is rapidly produced in response to other cell stress agents with no direct relation to virus such as bacterial lipopolysaccharide and interleukin-1, but its function under these non-viral infection cell stress conditions has yet to be elucidated. Here, we demonstrate an interaction between IFIT1 and eukaryotic elongation factor-1A (eEF1A) both in vitro, using recombinant proteins as bait in pull-down assays, and in vivo, using laser confocal microscopy and immunoprecipitation. In addition, we report the initial determination of the domain of IFIT1 that mediates this interaction. We also display that both IFIT1 and eEF1A protein levels are rapidly elevated, prolonged in tumor necrosis factor alpha pre-treated Raw264.7 cells, and most of those cells are induced to death by the end of investigations. Our results imply that under some stressful stimulations IFIT1 may participate in cell death pathways by interaction with eEF1A.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Wathelet M, Moutschen S, Defilippi P, Cravador A, Collet M, Huez G, Content J (1986) Molecular cloning, full-length sequence and preliminary characterization of a 56-kDa protein induced by human interferons. Eur J Biochem 155:11–17. doi:10.1111/j.1432-1033.1986.tb09452.x

    Article  CAS  PubMed  Google Scholar 

  2. Smith JB, Herschman HR (1996) The glucocorticoid attenuated response genes GARG-16, GARG-39, and GARG-49/IRG2 encode inducible proteins containing multiple tetratricopeptide repeat domains. Arch Biochem Biophys 330:290–300. doi:10.1006/abbi.1996.0256

    Article  CAS  PubMed  Google Scholar 

  3. Sikorski RS, Boguski MS, Goebl M, Hieter P (1990) A repeating amino acid motif in CDC23 defines a family of proteins and a new relationship among genes required for mitosis and RNA synthesis. Cell 60:307–317. doi:10.1016/0092-8674(90)90745-Z

    Article  CAS  PubMed  Google Scholar 

  4. Sen GC (2000) Novel functions of interferon-induced proteins. Semin Cancer Biol 10:93–101. doi:10.1006/scbi.2000.0312

    Article  CAS  PubMed  Google Scholar 

  5. Bluyssen HA, Vlietstra RJ, Faber PW, Smit EM, Hagemeijer A, Trapman J (1994) Structure, chromosome localization, and regulation of expression of the interferon-regulated mouse Ifi54/Ifi56 gene family. Genomics 24:137–148. doi:10.1006/geno.1994.1591

    Article  CAS  PubMed  Google Scholar 

  6. Ye S, Pang H, Gu YY, Hua J, Chen XG, Bao CD, Wang Y, Zhang W, Qian J, Tsao BP, Hahn BH, Chen SL, Rao ZH, Shen N (2003) Protein interaction for an interferon-inducible systemic lupus associated gene, IFIT1. Rheumatology 42:1155–1163. doi:10.1093/rheumatology/keg315

    Article  CAS  PubMed  Google Scholar 

  7. Guo J, Peters KL, Sen GC (2000) Induction of the human protein P56 by interferon, double-stranded RNA, or virus infection. Virology 267:209–219. doi:10.1006/viro.1999.0135

    Article  CAS  PubMed  Google Scholar 

  8. Guo J, Hui DJ, Merrick WC, Sen GC (2000) A new pathway of translational regulation mediated by eukaryotic initiation factor 3. EMBO J 19:6891–6899. doi:10.1093/emboj/19.24.6891

    Article  CAS  PubMed  Google Scholar 

  9. Guo J, Sen GC (2000) Characterization of the interaction between the interferon-induced protein P56 and the Int6 protein encoded by a locus of insertion of the mouse mammary tumor virus. J Virol 74:1892–1899. doi:10.1128/JVI.74.4.1892-1899.2000

    Article  CAS  PubMed  Google Scholar 

  10. Wathelet MG, Clauss IM, Nols CB, Content J, Huez GA (1987) New inducers revealed by the promoter sequence analysis of two interferon-activated human genes. Eur J Biochem 169:313–321. doi:10.1111/j.1432-1033.1987.tb13614.x

    Article  CAS  PubMed  Google Scholar 

  11. Smith JB, Herschman HR (1995) Glucocorticoid-attenuated response genes encode intercellular mediators, including a new C-X-C chemokine. J Biol Chem 270:16756–16765. doi:10.1074/jbc.270.28.16756

    Article  CAS  PubMed  Google Scholar 

  12. Browne GJ, Proud CG (2002) Regulation of peptide-chain elongation in mammalian cells. Eur J Biochem 269:5360–5368. doi:10.1046/j.1432-1033.2002.03290.x

    Article  CAS  PubMed  Google Scholar 

  13. Lamberti A, Caraglia M, Longo O, Marra M, Abbruzzese A, Arcari P (2004) The translation elongation factor 1A in tumorigenesis, signal transduction and apoptosis: review article. Amino Acids 26:443–448. doi:10.1007/s00726-004-0088-2

    Article  CAS  PubMed  Google Scholar 

  14. Chen E, Proestou G, Bourbeau D, Wang E (2000) Rapid up-regulation of peptide elongation factor ef-1a protein levels is an immediate early event during oxidative stress-induced apoptosis. Exp Cell Res 259:140–148. doi:10.1006/excr.2000.4952

    Article  CAS  PubMed  Google Scholar 

  15. Li L, Elledge SJ, Peterson CA, Bales ES, Legerski RJ (1994) Specific association between the human DNA repair proteins XPA and ERCC1. Proc Natl Acad Sci USA 91(11):5012–5016. doi:10.1073/pnas.91.11.5012

    Article  CAS  PubMed  Google Scholar 

  16. Donovan J, Brown P (1995) Current protocols in immunology. Wiley, New York

    Google Scholar 

  17. Shankar SL, O’Guin K, Kim M, Varnum B, Lemke G, Brosnan CF, Shafit-Zagardo B (2006) Gas6/Axl signaling activates the phosphatidylinositol 3-kinase/Akt1 survival pathway to protect oligodendrocytes from tumor necrosis factor alpha-induced apoptosis. J Neurosci 26:5638–5648. doi:10.1523/JNEUROSCI.5063-05.2006

    Article  CAS  PubMed  Google Scholar 

  18. Hui DJ, Bhasker CR, Merrick WC, Sen GC (2003) Viral stress-inducible protein p56 inhibits translation by blocking the interaction of eIF3 with the ternary complex eIF2.GTP.Met-tRNAi. J Biol Chem 278:39477–39482. doi:10.1074/jbc.M305038200

    Article  CAS  PubMed  Google Scholar 

  19. Hui DJ, Terenzi F, Merrick WC, Sen GC (2004) Mouse p56 blocks a distinct function of eukaryotic initiation factor 3 in translation initiation. J Biol Chem 280:3433–3440. doi:10.1074/jbc.M406700200

    Article  PubMed  Google Scholar 

  20. Sarkar SN, Sen GC (2004) Novel functions of proteins encoded by viral stress-inducible genes. Pharmacol Ther 103:245–259. doi:10.1016/j.pharmthera.2004.07.007

    Article  CAS  PubMed  Google Scholar 

  21. D’ Andrea LD, Regan L (2003) TPR protein: the versatile helixes. Trends Biochem Sci 28:655–662. doi:10.1016/j.tibs.2003.10.007

    Article  Google Scholar 

  22. Das AK, Cohen PW, Barford D (1998) The structure of the tetratricopeptide repeats of protein phosphatase 5: implications for TPR-mediated protein-protein interactions. EMBO J 17:1192–1199. doi:10.1093/emboj/17.5.1192

    Article  CAS  PubMed  Google Scholar 

  23. Condeelis J (1995) Elongation factor 1 alpha, translation and the cytoskeleton. Trends Biochem Sci 20:169–170. doi:10.1016/S0968-0004(00)88998-7

    Article  CAS  PubMed  Google Scholar 

  24. Duttaroy A, Bourbeau D, Wang XL, Wang E (1998) Apoptosis rate can be accelerated or decelerated by overexpression or reduction of the level of elongation factor-1a. Exp Cell Res 238:168–176. doi:10.1006/excr.1997.3819

    Article  CAS  PubMed  Google Scholar 

  25. Lund A, Knudsen SM, Vissing H, Clark B, Tommerup N (1996) Assignment of human elongation factor 1alpha genes: EEF1A maps to chromosome 6q14 and EEF1A2 to 20q13.3. Genomics 36:359–361. doi:10.1006/geno.1996.0475

    Article  CAS  PubMed  Google Scholar 

  26. Kahns S, Lund A, Kristensen P, Knudsen CR, Clark BF, Cavallius J, Merrick WC (1998) The elongation factor1 A-2 isoform from rabbit cloning of the cDNA and characterization of the protein. Nucleic Acids Res 26:1884–1890. doi:10.1093/nar/26.8.1884

    Article  CAS  PubMed  Google Scholar 

  27. Ruest LB, Marcotte R, Wang E (2002) Peptide elongation factor eEF1A–2/S1 expression in cultured differentiated myotubes and its protective effect against caspase3 mediated apoptosis. J Biol Chem 277:5418–5425. doi:10.1074/jbc.M110685200

    Article  CAS  PubMed  Google Scholar 

  28. Borradaile NM, Buhman KK, Listenberger LL, Magee CJ, Morimoto ET, Ory DS, Schaffer JE (2005) A critical role for eukaryotic elongation factor 1A-1 in lipotoxic cell death. Mol Biol Cell 17:770–778. doi:10.1091/mbc.E05-08-0742

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We are grateful to Ms. Liu Jin-Xiu for her help in mass spectrum analysis, to Ms. Shun Wei for her help in laser confocal microscopic observation, and to Dr. Yan Guo-He for her help in conducting cell transfections. This study was supported by grants from the National Key Basic Research and Development Plan of China (“973” projects. G1999054201 and 2005CB522605), and by grants from the Cooperative Research Foundation for Oversea Young Scholar of China (30328025).

Author information

Authors and Affiliations

  1. State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China

    Hong-Tao Li, Yong-Ping Su, Tian-Min Cheng, Guo-Ping Ai & Jun-Ping Wang

  2. Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA

    Jian-Ming Xu & Jie Liao

  3. Department of Otolaryngology Head and Neck Surgery, Daping Hospital Institute of Surgery Research, Third Military Medical University, Chongqing, 400042, China

    Hong-Tao Li, Ji-Chuan Chen & Chang-You Ji

Authors
  1. Hong-Tao Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yong-Ping Su
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tian-Min Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jian-Ming Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jie Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ji-Chuan Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Chang-You Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Guo-Ping Ai
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jun-Ping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hong-Tao Li.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, HT., Su, YP., Cheng, TM. et al. The interaction between interferon-induced protein with tetratricopeptide repeats-1 and eukaryotic elongation factor-1A. Mol Cell Biochem 337, 101–110 (2010). https://doi.org/10.1007/s11010-009-0289-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11010-009-0289-9

Keywords

Search

Navigation