Abstract
Histamine is a potent mediator in allergic inflammatory processes and is released by basophils and mast cells. The aim of this study was to investigate the effect of histamine on in vitro migration of human fetal lung fibroblasts (HFL-1) to human plasma fibronectin (HFn), a chemoattractant. Using the blindwell chamber technique, histamine alone had no chemotactic activity. However, histamine augmented HFn-induced HFL-1 migration at concentrations ranging between 0 and 10−7 M (290.6 ± 20.8%) (P < 0.05). The concentration-response was bell-shaped. The effect of histamine increased with time. The stimulatory effect of histamine on HFL-1 migration was inhibited by an H4 receptor antagonist, JNJ7777120 (10−5 M). Histamine’s effect was also inhibited by pertussis toxin (50 ng/ml), showing that the effect was mediated by the H4 receptor. This study demonstrated that histamine has the potential to stimulate human lung fibroblast migration, and thus may contribute to regulation of wound healing and the development of fibrotic disorders of the lung.
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Acknowledgements
This work was supported in part by grants-in-aid for Scientific Research from the Ministry of Education, Science, Sports, Culture and Technology of Japan, a grant to the Respiratory Failure Research Group from the Ministry of Health, Labour and Welfare, Japan, and grants-in-aid for Comprehensive Research on Aging and Health from the Ministry of Health, Labour and Welfare, Japan. The authors thank Miss Makiko Kase for her excellent technical assistance.
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Kohyama, T., Yamauchi, Y., Takizawa, H. et al. Histamine stimulates human lung fibroblast migration. Mol Cell Biochem 337, 77–81 (2010). https://doi.org/10.1007/s11010-009-0287-y
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DOI: https://doi.org/10.1007/s11010-009-0287-y