Abstract
Adipose triglyceride lipase (ATGL), as an adipose-enriched protein, is able to hydrolyze triglycerides and plays an important part in triglyceride lipolysis of fat tissue. Leptin, an adipocyte cytokine, can increase the fat decomposition process. Many phenomena indicate that ATGL has a close relationship with leptin’s promoting the hydrolysis of triglycerides. However, the regulatory mechanism of ATGL in leptin’s promoting fat hydrolysis has not been directly and systematically studied yet. This study demonstrated that ATGL was expressed in vitro by leptin regulation. The amount of ATGL mRNA increased and the amount of ATGL protein decreased based on a dose-dependent manner when leptin concentrations ranged from 5 to 50 ng/ml were used to treat fully differentiated porcine adipocytes for 3 h. In addition, this study revealed that JAK-STAT and MAPK signaling pathways, as well as PPARγ all played important roles in the ATGL expression mediated by leptin.
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Acknowledgments
We are grateful to Dr Bin Wu for critical reading and revising this manuscript and English correction. Also, authors thank Dr Gui-bao Fan for review of the manuscript. This study was supported by grant of the National High Technology Research and Development Program of China (863 Program; No. 2006AA10Z138).
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Li, Yc., Zheng, Xl., Liu, Bt. et al. Regulation of ATGL expression mediated by leptin in vitro in porcine adipocyte lipolysis. Mol Cell Biochem 333, 121–128 (2010). https://doi.org/10.1007/s11010-009-0212-4
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DOI: https://doi.org/10.1007/s11010-009-0212-4