Abstract
The economic damage caused by H5N1 avian influenza virus outbreak in domestic poultry and the threat of this virus to human health make the research of this virus highly significant. During the 2004 outbreak of avian influenza in Hubei province, People’s Republic of China, we isolated a new H5N1 subtype avian influenza virus named as A/chicken/Hubei/489/2004(H5N1) (shorten as AIVHubei489 here). In this study, the infectivity and apoptosis-inducing characteristics of AIVHubei489 were studied. We demonstrated that AIVHubei489 could infect MDCK cells and the infection induced apoptosis. Our data also showed that the apoptosis induced by this virus in MDCK cells was caspase activity dependent. Moreover, we proved that caspase 8 but not caspase 9 was involved in this apoptosis. The infectivity and apoptosis-inducing activity of AIVHubei489 in Vero and HeLa cells were also studied. Our results showed that AIVHubei489 could replicate in Vero and HeLa cells, but the infection did not cause apoptosis in either of the two cell lines. Thus, AIVHubei489 induced apoptosis through a caspase-dependent pathway in a cell-specific manner.
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References
World Health Organization (2007) H5N1 avian influenza: timeline of major events http://www.who.int/csr/disease/avian_influenza/timeline2007_06_21.pdf
World Health Organization (2007) Cumulative number of confirmed human cases of avian influenza A/(H5N1) Reported to WHO 25 June 2007 http://www.who.int/csr/disease/avian_influenza/country/cases_table_2007_06_25/en/index.html
Govorkova EA, Murti G, Meignier B, Taisne C, Webster RG (1996) African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. J Virol 551:9–5524
Brydon EW, Morris SJ, Sweet C (2005) Role of apoptosis and cytokines in influenza virus morbidity. FEMS Microbiol Rev 29:837–850
Fesq H, Nain M, Nain M (1994) Programmed cell death (apoptosis) in human monocytes infected by influenza A virus. Immunobiology 190:175–182
Hinshaw VS, Olsen CW, Dybdahl-Sissoko N, Evans D (1994) Apoptosis: a mechanism of cell killing by influenza A and B viruses. J Virol 366:7–3673
Mori I, Komatsu T, Takeuchi K, Nakakuki K, Sudo M, Kimura Y (1995) In vivo induction of apoptosis by influenza virus. J Gen Virol 76:2869–2873
Price GE, Smith H, Sweet C (1997) Differential induction of cytotoxicity and apoptosis by influenza Virus strains of differing virulence. J Gen Virol 78:2821–2829
Takizawa T, Matsukawa S, Higuchi Y, Nakamura S, Nakanishi Y, Fukuda R (1993) Induction of programmed cell death (apoptosis) by influenza virus infection in tissue culture cells. J Gen Virol 74:2347–2355
Takizawa T, Tatematsu C, Ohashi K, Nakanishi Y (1999) Recruitment of apoptotic cysteine proteases (caspases) in influenza virus-induced cell death. Microbiol Immunol 43:245–252
Wurzer WJ, Planz O, Ehrhardt C, Giner M, Silberzahn T, Pleschka S, Ludwig S (2003) Caspase 3 activation is essential for efficient influenza virus propagation. EMBO J 22:2717–2728
Ashkenazi A, Dixit VM (1998) Death receptors: signaling and modulation. Science 281:1305–130820
Green DR, Reed JC (1998) Mitochondria and apoptosis. Science 281:1309–1312
Thornberry NA, Lazebnik Y (1998) Caspases: enemies within. Science 81:1312–1316
Liu T, Brouha B, Grossman D (2004) Rapid induction of mitochondrial events and caspase-independent apoptosis in survivin-targeted melanoma cells. Oncogene 23:39–48
Ota E, Nagashima Y, Shiomi K, Sakurai T, Kojima C, Waalkes MP, Himeno S (2006) Caspase-independent apoptosis induced in rat liver cells by plancitoxin I, the major lethal factor from the crown-of-thorns starfish Acanthaster planci venom. Toxicon 48:1002–1010
Lin C, Holland RE, Donofrio JC, McCoy MH, Tudor LR, Chambers TM (2002) Caspase activation in equine influenza virus induced apoptotic cell death. Vet Microbiol 84:357–365
Qu SF, Zheng CY, Hu GB, Peng F, Ruan YH (1999) Programmed cell death induced by ultraviolet irradiation in the cell lines of kidney from grass carp. Chin J Fish 12:42–46
Choi YK, Kim TK, Kim CJ, Lee JS, Oh SY, Joo HS, Foster DN, Hong KC, You S, Kim H (2006) Activation of the intrinsic mitochondrial apoptotic pathway in swine influenza virus-mediated cell death. Exp Mol Med 38:11–17
Balachandran S, Roberts PC, Kipperman T, Bhalla KN, Compans RW, Archer DR, Barber GN (2000) Alpha/beta interferons potentiate virus-induced apoptosis through activation of the FADD/caspase-8 death signaling pathway. J Virol 74:1513–1523
Mok CK, Lee DC, Cheung CY, Peiris M, Lau AS (2007) Differential onset of apoptosis in influenza A virus H5N1- and H1N1-inducted human blood macrophages. J Gen Virol 88:1275–1280
Acknowledgements
This study was supported by three Grants as follows: 973 Program (No. 2006CB504300), Infrastructure and Facility Development Program (No. 2004DKA305400), and National Science Foundation Program of Hubei Province (No. 2006ABA230).
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Yang, W., Qu, S., Liu, Q. et al. Avian influenza virus A/chicken/Hubei/489/2004 (H5N1) induces caspase-dependent apoptosis in a cell-specific manner. Mol Cell Biochem 332, 233–241 (2009). https://doi.org/10.1007/s11010-009-0196-0
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DOI: https://doi.org/10.1007/s11010-009-0196-0