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Alternative Fas-mediated cell death pathway is dependent on the different cleavage patterns of procaspase-8

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Abstract

It has been reported that mitochondria-independent or mitochondria-dependent (type I/II) Fas signaling pathways in leukemia cells depend on the amount of active caspase-8. However, Bid molecules, which could not be cleaved in type I cells, could be effectively cleaved by recombinant active caspase-8 in vitro. The cleavage of recombinant Bid by recombinant active caspase-8 could be blocked by anti-p10 and anti-p18 specific antibodies. Fas receptors could be similarly internalized into cytoplasm in type I and type II cells. Interestingly, p10 subunit of active caspase-8 could be detected in both type I and II cells, while p18 subunit of active caspase-8 could be detected only in type II cells but not in type I cells. These results demonstrated that p18 subunit was necessary for Bid cleavage and the mitochondria pathway might be dependent on the release of p18 subunit from active caspase-8.

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Acknowledgment

This study was supported in part by Grants from the Ministry of Education, Science, Sports and Culture, Japan.

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Authors and Affiliations

  1. Department of Pediatric and Developmental Science, Mie University Graduate School of Medicine, Edobashi 2-174, Tsu, Mie, 514-8507, Japan

    Yufeng Li, Lei Qi, Atsushi Iwao, Kentaro Kihira, Francis Dida, Eiichi Azuma & Yoshihiro Komada

  2. Department of Molecular Pathobiology, Mie University Graduate School of Medicine, Tsu, Mie, Japan

    Zhenhu Song

  3. Division of Cell Transplantation and Therapy, Mie University School of Medicine, Tsu, Mie, Japan

    Eiichi Azuma & Yoshihiro Komada

  4. Xinhua Hospital Affiliated to Shanghai JiaoTong University School of Medicine, Shanghai, China

    Yufeng Li & Lei Qi

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  1. Yufeng Li
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  2. Lei Qi
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Correspondence to Yoshihiro Komada.

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Li, Y., Qi, L., Iwao, A. et al. Alternative Fas-mediated cell death pathway is dependent on the different cleavage patterns of procaspase-8. Mol Cell Biochem 331, 231–238 (2009). https://doi.org/10.1007/s11010-009-0164-8

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  • DOI: https://doi.org/10.1007/s11010-009-0164-8

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