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Monitor of the myostatin autocrine action during differentiation of embryonic chicken myoblasts into myotubes: effect of IGF-I

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Abstract

Myogenesis is regulated through the proliferation and differentiation of myoblasts expressing myostatin which functions as a negative regulator by generating Smad signals. Here, we monitored the autocrine action of myostatin in quiescent chicken myoblasts transfected with the Smad-mediated promoter reporter vector to evaluate the modulation of several growth factors. During differentiation of myoblasts into myotubes, stretched and spherical types of myoblasts were observed at 12 h after induction, at which the promoter activity began to increase. Maximal promoter activity was observed at approximately 30 h. Multinucleated myotubes were markedly formed at 72 h, but the activity was very low. IGF-I, known as a positive regulator of myogenesis, increased the promoter activity, but the increase was rather small at its high concentration (100 ng/ml). IGF-I significantly increased the level of myostatin transcript in myoblasts and newly formed myotubes at 24 h, but not at 36 h. However, the cell fusion of myoblasts was not accelerated in the presence of IGF-I. Consequently, this study indicates that the autocrine action of myostatin is partially enhanced by IGF-I through increasing its expression.

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Acknowledgments

This research was supported in part by a Grant-in-Aid for Scientific Research (B) from the Japan Society for the Promotion of Sciences (JSPS; 16380200) (to M.-A. Hattori). F. Sato and S. Aramaki were, respectively, supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (No. 166796 and No. 09744).

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Authors and Affiliations

  1. Laboratory of Reproductive Physiology and Biotechnology, Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Masatoshi Kurokawa, Fuminori Sato, Shinya Aramaki, Tomoki Soh, Nobuhiko Yamauchi & Masa-aki Hattori

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  1. Masatoshi Kurokawa
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  2. Fuminori Sato
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  3. Shinya Aramaki
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  6. Masa-aki Hattori
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Correspondence to Masa-aki Hattori.

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Kurokawa, M., Sato, F., Aramaki, S. et al. Monitor of the myostatin autocrine action during differentiation of embryonic chicken myoblasts into myotubes: effect of IGF-I. Mol Cell Biochem 331, 193–199 (2009). https://doi.org/10.1007/s11010-009-0158-6

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  • DOI: https://doi.org/10.1007/s11010-009-0158-6

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