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Survivin is involved in the anti-apoptotic effect of edaravone in PC12 cells

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Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one), a newly developed hydroxy radical scavaging agent which has been widely used for protection against ischemia-reperfusion injury is highly effective in preventing cell apoptosis. However, the exact intracellular mechanism(s) underlying the protective action of edaravone is not clear. We observed that in PC12 cells cultured under serum deprivation (DEPV) condition, the levels of survivin were positively correlated with the anti-apoptotic action of edaravone. Survivin RNA interference (RNAi) increased DEPV-induced PC12 cell apoptosis, whereas the anti-apoptotic effect of edaravone was blunted by survivin RNAi. Moreover, survivin overexpression provided protection against DEPV-induced PC12 cell apoptosis. Inhibition of ERK and PI3-K/AKT prevented edaravone’s ability to decrease apoptosis and increase survivin. In conclusion, the present study provides the first direct evidence that survivin involves in the anti-apoptotic effects of edaravone via a pathway involving ERK and PI3-K/AKT.

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This study was supported by grants from the Foundation of Science and Technology Commission of Shanghai Municipality (04ZR14021) and the Foundation of Excellent Youth Scientist of Tongji University.

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Correspondence to Yong-Gang Wang.

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Xue-Yuan Liu and Ling-Ling Yao have contributed equally to this study.

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Liu, XY., Yao, LL., Chen, YJ. et al. Survivin is involved in the anti-apoptotic effect of edaravone in PC12 cells. Mol Cell Biochem 327, 21–28 (2009).

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