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Expression of fasciculation and elongation protein zeta-1 (FEZ1) in cultured rat neonatal astrocytes

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Abstract

Astrocytes play a more important role than simply providing physical support for neurons, however, the function(s) of type 1 and type 2 astrocytes (T1As, T2As), remains unclear. A DNA microarray was used to identify gene expression in cultured T1As and T2As isolated from postnatal day 1 rat cortex. Ninety-nine of the 138 differentially expressed genes were involved in a diverse number of processes. The fasciculation and elongation protein zeta-1 (FEZ1) gene was studied further because it has been suggested that it is not expressed by astrocytes. RT-PCR and Western blots confirmed the microarray data and showed that FEZ1 was present in T1 and T2As and is more highly expressed in T2As. Immunocytochemistry revealed that FEZ1 was located in the astrocytic cytoplasm and cell processes but not the nucleus. The results contribute to a clearer understanding of the two types of astrocytes.

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Acknowledgments

This study was supported by National Natural Science Foundation of China (Grant No. 30772242). The authors thank Dr. T. FitzGibbon for comments on earlier drafts of the manuscript.

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Authors and Affiliations

  1. Cytoneurobiology Unit & Laboratory of Aging and Nervous Diseases, Medical College of Soochow University, 199 Ren-Ai Road, Suzhou Industrial Park, Suzhou, JS, 215123, People’s Republic of China

    Jianghong He, Jun Liu, Maomin Sun & Chunlin Xia

  2. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS, 226001, People’s Republic of China

    Jianghong He, Zhijun Zhang & Ting Zhu

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  1. Jianghong He
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  2. Jun Liu
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  3. Zhijun Zhang
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  4. Maomin Sun
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  6. Chunlin Xia
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Correspondence to Chunlin Xia.

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He, J., Liu, J., Zhang, Z. et al. Expression of fasciculation and elongation protein zeta-1 (FEZ1) in cultured rat neonatal astrocytes. Mol Cell Biochem 325, 159–167 (2009). https://doi.org/10.1007/s11010-009-0030-8

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  • DOI: https://doi.org/10.1007/s11010-009-0030-8

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