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Differential activation of mitogen-activated protein kinases following high and low LET radiation in murine macrophage cell line

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Abstract

Mitogen-activated protein kinases have been shown to respond to various stimuli including cytokines, mitogens and gamma irradiation, leading to cell proliferation, differentiation, or death. The duration of their activation determines the specificity of response to each stimulus in various cells. In this study, the crucial intracellular kinases, ERK, JNK, and p38 kinase involved in cell survival, death, or damage and repair were examined for their activity in RAW 264.7 cells at various time points after irradiation with 2 Gy doses of proton ions or X-rays. This is the first report that shows that the MAPK signaling induced after heavy ion or X-ray exposure is not the same. Unlike gamma irradiation, there was prolonged but marginal activation of prosurvival ERK pathway and significant activation of proapoptotic p38 pathway in response to high LET radiation.

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Authors and Affiliations

  1. Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India

    Himanshi Narang & Malini Krishna

  2. Radiological Protection and Dosimetry Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India

    Nagesh Bhat

  3. Nuclear Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India

    S. K. Gupta, S. Santra, R. K. Choudhary & S. Kailash

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  1. Himanshi Narang
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Correspondence to Malini Krishna.

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Narang, H., Bhat, N., Gupta, S.K. et al. Differential activation of mitogen-activated protein kinases following high and low LET radiation in murine macrophage cell line. Mol Cell Biochem 324, 85–91 (2009). https://doi.org/10.1007/s11010-008-9987-y

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  • DOI: https://doi.org/10.1007/s11010-008-9987-y

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