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Mipu1, a novel rat zinc-finger protein, inhibits transcriptional activities of AP-1 and SRE in mitogen-activated protein kinase signaling pathway

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Abstract

Mipu1 is a novel rat gene recently identified in our lab. Mipu1 cDNA contains a 1,824 bp open reading frame (ORF) and encoded a 608 amino acid protein with an N-terminal Krüppel-associated box (KRAB) domain and classical zinc finger C2H2 motifs in the C-terminus. Mipu1 protein is located in the nuclei. Fused to Gal-4 DNA-binding domain and cotransfected with pG5-luc, Mipu1 played a transcriptional suppressive effect. Deletion analysis with a series of truncated fusion proteins indicated that the KRAB motif was a basal repression domain. Overexpression of Mipu1 in H9c2 myogenic cells inhibited the transcriptional activities of SRE and AP-1. RNAi of Mipu1 in H9c2 myogenic cells activated the transcriptional activities of SRE and AP-1. These results suggested that Mipu1 protein might act as a transcriptional repressor in mitogen-activated protein kinase (MAPK) signaling pathway to mediate cellular functions.

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Acknowledgments

This work was supported by funding from the National Natural Science Foundation of China (30770855, 30672149), the Major National Basic Research Program of China (2007CB512007).

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Authors and Affiliations

  1. Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan, 410008, People’s Republic of China

    Guiliang Wang, Can Yuan, Yazhu Zheng, Lei Jiang, Juan Song, Ying Liu, Bin Zhang & Xianzhong Xiao

  2. Department of Digestive Internal Medicine, People’s Hospital of Pingxiang City, Pingxiang, Jiangxi, 337055, People’s Republic of China

    Guiliang Wang

  3. Department of Rheumatology and Clinical Immunology, Xiangya Hospital, Central South University, Changsha, 410008, People’s Republic of China

    Xiaoxia Zuo

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  1. Guiliang Wang
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  2. Xiaoxia Zuo
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  3. Can Yuan
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  4. Yazhu Zheng
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  5. Lei Jiang
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  9. Xianzhong Xiao
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Corresponding author

Correspondence to Xianzhong Xiao.

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Guiliang Wang and Xiaoxia Zuo contributed equally to this work.

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Wang, G., Zuo, X., Yuan, C. et al. Mipu1, a novel rat zinc-finger protein, inhibits transcriptional activities of AP-1 and SRE in mitogen-activated protein kinase signaling pathway. Mol Cell Biochem 322, 93–102 (2009). https://doi.org/10.1007/s11010-008-9944-9

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  • DOI: https://doi.org/10.1007/s11010-008-9944-9

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