Skip to main content

Advertisement

SpringerLink
Log in

Enhanced migration and CXCR4 over-expression in fibroblasts with telomerase reconstitution

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Telomerase reconstitution shows great potential for cell treatment and tissue engineering. Although the effects of telomerase on cell lifespan are well documented, the effects of telomerase on cellular biological characteristics, such as cellular migration, are relatively unknown. In this study, we tried to investigate if telomerase is involved in the regulation of fibroblast migration and the mechanism behind it. We found that when stimulated with a chemokine, CXCL12, the rate of migration was significantly higher in fibroblasts with telomerase reconstitution than that in fibroblasts without. Furthermore, the CXCL12 receptor, CXCR4, and multiple down-stream factors (Rho family members), were upregulated in the telomerase reconstituted fibroblasts. We concluded for the first time that telomerase reconstitution increased fibroblast migration through activation of CXCL12/CXCR4 axis and Rho family. The finding that fibroblasts with telomerase reconstitution have enhanced migration may have broad implications for cell therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Bodnar AG, Ouellette M , Frolkis M et al (1998) Extension of life-span by introduction of telomerase into normal human cells. Science 279:349–352

    Article  PubMed  CAS  Google Scholar 

  2. Shay JW, Wright WE (2005) Use of telomerase to create bioengineered tissues. Ann N Y Acad Sci 1057:479–491

    Article  PubMed  CAS  Google Scholar 

  3. Murasawa S, Llevadot J, Silver M et al (2002) Constitutive human telomerase reverse transcriptase expression enhances regenerative properties of endothelial progenitor cells. Circulation 106:1133–1139

    Article  PubMed  CAS  Google Scholar 

  4. Sato N, Maehara N, Mizumoto K et al (2001) Telomerase activity of cultured human pancreatic carcinoma cell lines correlates with their potential for migration and invasion. Cancer 91:496–504

    Article  PubMed  CAS  Google Scholar 

  5. Claps CM, Corcoran KE, Cho KJ et al (2005) Stromal derived growth factor-1alpha as a beacon for stem cell homing in development and injury. Curr Neurovasc Res 2:319–329

    Article  PubMed  CAS  Google Scholar 

  6. Busillo JM, Benovic JL (2007) Regulation of CXCR4 signaling. Biochim Biophys Acta 1768:952–963

    Article  PubMed  CAS  Google Scholar 

  7. Caruz A , Samsom M, Alonso JM et al (1998) Genomic organization and promoter characterization of human CXCR4 gene. FEBS Lett 426:271–278

    Article  PubMed  CAS  Google Scholar 

  8. Wegner SA, Ehrenberg PK, Chang G et al (1998) Genomic organization and functional characterization of the chemokine receptor CXCR4, a major entry co-receptor for human immunodeficiency virus type 1. J Biol Chem 273:4754–4760

    Article  PubMed  CAS  Google Scholar 

  9. Tan W, Martin D, Gutkind JS (2006) The Gα13-Rho signaling axis is required for SDF-1-induced migration through CXCR4. J Biol Chem 28:39542–39549

    Article  Google Scholar 

  10. Xie HQ, Yang ZM, Qu Y (2002) Culture of human fibroblasts transfected by human telomerase reverse transcriptase eukaryotic expression plasmid pGRN145 and their biological characteristics in vitro (in Chinese). Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 16:195–199

    PubMed  Google Scholar 

  11. Strutz F, Okada H, Lo CW et al (1995) Identification and characterization of a fibroblast marker: FSP1. J Cell Biol 130:393–405

    Article  PubMed  CAS  Google Scholar 

  12. Bagheri S, Nosrati M, Li S et al (2006) Genes and pathways downstream of telomerase in melanoma metastasis. Proc Natl Acad Sci U S A 103:11306–11311

    Article  PubMed  CAS  Google Scholar 

  13. Ridley AJ, Schwartz MA, Burridge K et al (2003) Cell migration: integrating signals from front to back. Science 302:1704–1709

    Article  PubMed  CAS  Google Scholar 

  14. Nobes CD, Hall A (1995) Rho, rac and Cdc42 GTPases: regulators of actin structures, cell adhesion and motility. Biochem Soc Trans 23:456–459

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30570623 and No. 30770748 to Dezhi Mu), Doctoral Program of Ministry of Education of China (No. 20050610094 to Yi Qu and No. 20070610092 to Dezhi Mu), China Medical Board of New York (00-722 to Dezhi Mu). We sincerely appreciate the help of Weiya Mu from Wellesley College for proof reading for the manuscript.

Author information

Authors and Affiliations

  1. Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, China

    Yi Qu, Meng Mao, Xihong Li, Fengyang Zhao, Ying Xiong & Dezhi Mu

  2. West China Medical Center, Sichuan University, Chengdu, 610041, China

    Lin Zhang & Chunlei Yang

  3. Department of Urology, The People’s Hospital of Sichuan Province, Chengdu, 610072, China

    Xiang Huang

Authors
  1. Yi Qu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Meng Mao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xihong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiang Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chunlei Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Fengyang Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ying Xiong
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Dezhi Mu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dezhi Mu.

Additional information

Yi Qu and Meng Mao, contributed equally to this report.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Qu, Y., Mao, M., Li, X. et al. Enhanced migration and CXCR4 over-expression in fibroblasts with telomerase reconstitution. Mol Cell Biochem 313, 45–52 (2008). https://doi.org/10.1007/s11010-008-9740-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11010-008-9740-6

Keywords

Search

Navigation