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I-FABP expression alters the intracellular distribution of the BODIPY C16 fatty acid analog

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Abstract

To investigate the structure–function relationships of intestinal fatty acid-binding protein (I-FABP) in cellular fatty acid (FA) trafficking, we compared the distribution of a fluorescent FA analog (BODIPY FL C16) in Cos-1 cells transiently transfected with the wild type protein (wt I-FABP) to that of a variant deleted of the alpha helical domain (HL I-FABP). In vector-only cells, BODIPY fluorescence was distributed throughout the cytoplasm. In the absence of added FA, wt I-FABP was found largely in the perinuclear region with some cytoplasmic staining as well. Addition of BODIPY FL C16 to transfected cells showed that the fluorescent FA was essentially completely colocalized with the protein in the cytoplasmic and perinuclear regions as well as in cytoplasmic clusters that are not observed in the absence of wt I-FABP. For HL I-FABP, the distribution of the protein in the absence of FA was diffusely cytoplasmic, in marked contrast to the wt protein. Addition of BODIPY led to less extensive colocalization than that observed for wt I-FABP. In particular, no localization to the perinuclear region was found. Organelle colocalization studies showed that both proteins colocalized with mitochondria and endoplasmic reticulum/golgi markers, but little with a lysosomal marker. The perinuclear localization for wt I-FABP and BODIPY did not show colocalization with any of the markers tested. Taken together, these results indicate that I-FABP binds FA in vivo and that the helical domain may be important for targeting I-FABP to a perinuclear domain but not, perhaps, to the endoplasmic reticulum, golgi apparatus or mitochondria.

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Abbreviations

BODIPY FL C16:

4,4-difluoro-5,7-dimethyl-4-bora-3a, 4adiaza-s-indacene-3-hexadecanoic acid

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Acknowledgments

J.S. was the recipient of a Poste-Orange fellowship from INSERM (National Institute of Health and Medical Research). This work was supported in part by U.S. Public Health Service NIH grant DK38389 (J.S.).

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Authors and Affiliations

  1. INSERM, U476 «Nutrition Humaine et Lipides», Marseille, 13385, France

    Julie Karsenty, Olfa Helal, Paulette Lechène de la Porte, Paule Beauclair-Deprez, Claire Martin-Elyazidi, Richard Planells & Marguerite Gastaldi

  2. INRA, UMR1260, Marseille, 13385, France

    Julie Karsenty, Olfa Helal, Paulette Lechène de la Porte, Paule Beauclair-Deprez, Claire Martin-Elyazidi, Richard Planells & Marguerite Gastaldi

  3. Faculté de Médecine, IPHM-IFR 125, Univ Méditerranée Aix-Marseille 2, Marseille, 13385, France

    Julie Karsenty, Olfa Helal, Paulette Lechène de la Porte, Paule Beauclair-Deprez, Claire Martin-Elyazidi, Richard Planells & Marguerite Gastaldi

  4. Department of Nutritional Sciences, School of Environmental and Biological Sciences, Rutgers University, 96 Lipman Drive, New Brunswick, NJ, 08901-8525, USA

    Judith Storch

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  1. Julie Karsenty
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Correspondence to Marguerite Gastaldi.

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Karsenty, J., Helal, O., Lechène de la Porte, P. et al. I-FABP expression alters the intracellular distribution of the BODIPY C16 fatty acid analog. Mol Cell Biochem 326, 97–104 (2009). https://doi.org/10.1007/s11010-008-0004-2

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