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Involvement of Annexin A2 in p53 induced apoptosis in lung cancer

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Abstract

Tumor suppressor p53 plays important roles in cell cycle regulation, apoptosis and DNA repair in different cell types including lung cancer. There are different p53 apoptotic pathways in high and low metastatic ability lung cancer cells. However, the exactly mechanism in the pathway is still unclear. Here we found that Annexin A2, a Ca2+-dependent phospholipid-binding protein, is involved in p53-mediated apoptosis. First, by using mRNA differential display technique, down-regulated Annexin A2 expression was found in all cell lines transfected of Ad-p53 (adenoviral expression construct encoding wild type p53 gene) especially in highly metastatic Anip973 lung cancer cells. Then, decreased expression of Annexin A2 was further confirmed by Northern blot and Western blot analysis. At last, knock down of Annexin A2 by siRNA inhibited cellular proliferation in BE1 cell line with highly metastatic ability. Taken together, our results suggested that Annexin A2 may play roles in p53 induced apoptosis and it is also involved in regulation of cell proliferation.

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Acknowledgements

This research was supported by the found (30471949) from National Natural Science Foundation of China; China Postdoctoral Science Foundation (20060400833;20060400834) and Postdoctoral Foundation of Heilongjiang Province.

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Authors and Affiliations

  1. Laboratory of Medical Genetics, Harbin Medical University, No. 194, Xuefu road, Nangang district, Harbin, Heilongjing Province, 150081, P.R. China

    Yun Huang, Yan Jin, Cheng-hui Yan, Yang Yu, Jing Bai, Feng Chen, Yu-zhen Zhao & Song-bin Fu

  2. Bio-pharmaceutical Key Laboratory of Heilongjiang Province, Harbin, 150081, P.R. China

    Song-bin Fu

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  1. Yun Huang
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  2. Yan Jin
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  3. Cheng-hui Yan
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  4. Yang Yu
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  5. Jing Bai
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  6. Feng Chen
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  7. Yu-zhen Zhao
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  8. Song-bin Fu
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Corresponding author

Correspondence to Song-bin Fu.

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The authors Yun Huang, Yan Jin and Cheng-hui Yan contributed equally to this work.

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Huang, Y., Jin, Y., Yan, Ch. et al. Involvement of Annexin A2 in p53 induced apoptosis in lung cancer. Mol Cell Biochem 309, 117–123 (2008). https://doi.org/10.1007/s11010-007-9649-5

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  • DOI: https://doi.org/10.1007/s11010-007-9649-5

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