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Hesperidin inhibits expression of hypoxia inducible factor-1 alpha and inflammatory cytokine production from mast cells

Abstract

The citrus unshiu peel has been used traditionally as a medicine to improve bronchial and asthmatic conditions or cardiac and blood circulation in Korea, China, and Japan. Here, we report the effects of citrus unshiu peel water extract (CPWE) on the phorbol myristate acetate (PMA) + calcium ionophore A23187-induced hypoxia-inducible factor-1α (HIF-1α) activation and inflammatory cytokine production from the human mast cell line, HMC-1 cells. We compared CPWE with hesperidin, a common constituent of citrus unshiu. CPWE and hesperidin inhibited the PMA + A23187-induced HIF-1α expression and the subsequent production of vascular endothelial growth factor (VEGF). In addition, CPWE suppressed PMA + A23187-induced phosphorylation of the extracellular signal-regulated kinase (ERK). We also show that the increased cytokines interleukin (IL)-1β, IL-8, and tumor necrosis factor (TNF)-α level was significantly inhibited by treatment of CPWE or hesperidin. In the present study, we report that CPWE and hesperidin are inhibitors of HIF-1α and cytokines on the mast cell-mediated inflammatory responses.

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Acknowledgment

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (R05-2003-000-11803-0).

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Correspondence to Jae-Young Um.

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Choi, IY., Kim, SJ., Jeong, HJ. et al. Hesperidin inhibits expression of hypoxia inducible factor-1 alpha and inflammatory cytokine production from mast cells. Mol Cell Biochem 305, 153–161 (2007). https://doi.org/10.1007/s11010-007-9539-x

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  • DOI: https://doi.org/10.1007/s11010-007-9539-x

Keywords

  • Citrus unshiu peel water extract
  • Hesperidin
  • Hypoxia-inducible factor-1α
  • Cytokines
  • Mast cells