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Lipopolysaccharide induces expression of SSeCKS in rat lung microvascular endothelial cell

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Abstract

Src-suppressed C kinase substrate (SSeCKS) plays a role in membrane-cytoskeletal remodeling to regulate mitogenesis, cell differentiation, and motility. Previous study showed that lipopolysaccharide (LPS) induced a selective and strong expression of SSeCKS in the vascular endothelial cells of lung. Here we show that LPS stimulation elevated expression of SSeCKS mRNA and protein in Rat pulmonary microvascular endothelial cell (RPMVEC). LPS potentiated SSeCKS phosphorylation in a time- and dose-dependent manner, and partly induced translocation of SSeCKS from the cytosol to the membrane after LPS challenge. The PKC inhibitor, Calphostin C, significantly decreased LPS-induced phosphorylation of SSeCKS, inhibited SSeCKS translocation and actin cytoskeleton reorganization after LPS challenge, suggesting that PKC may play a role in LPS-induced SSeCKS translocation and actin rearrangement. We conclude that SSeCKS is located downstream of PKC and that SSeCKS and PKC are both necessary for LPS-induced stress fiber formation.

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Acknowledgments

This work was supported by National Natural Scientific Foundation of China Grant 30300099, and Natural Science Foundation of JiangSu province Grant BK2003035, and Natural science Foundation of JiangSu colleges and universities Grant 03KJB180109 and “liu-da-ren-cai-gao-feng” finance assistance of JiangSu province Grant No. 2.

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Authors and Affiliations

  1. Institute of Nautical Medicine, Nantong University (former Nantong Medical College), Nantong, 226001, P.R. China

    Chun Cheng & Haiou Liu

  2. Medical College, Nantong University (former Nantong Medical College), Nantong, 226001, P.R. China

    Haiyan Ge, Ji Qian, Jing Qin, Linlin Sun & Menling Chen

  3. Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University (former Nantong Medical College), Nantong, 226001, P.R. China

    Meijuan Yan & Aiguo Shen

Authors
  1. Chun Cheng
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  2. Haiou Liu
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  3. Haiyan Ge
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  4. Ji Qian
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  5. Jing Qin
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  6. Linlin Sun
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  7. Menling Chen
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  8. Meijuan Yan
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  9. Aiguo Shen
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Correspondence to Aiguo Shen.

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Chun Cheng and Haiou Liu are contributed equally to this work.

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Cheng, C., Liu, H., Ge, H. et al. Lipopolysaccharide induces expression of SSeCKS in rat lung microvascular endothelial cell. Mol Cell Biochem 305, 1–8 (2007). https://doi.org/10.1007/s11010-007-9521-7

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  • DOI: https://doi.org/10.1007/s11010-007-9521-7

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