Abstract
An immunodominant heat shock protein (Hsp 24) was purified from Vibrio cholerae O139 at 42 °C and used as an immunomodulator for studying the gut immune response. T cell clone and T cell line specific for the Hsp 24 were generated from the lymphocytes of lamina propria and intra-epithelial lymphocytes of mice orally infected with V. cholerae O139, respectively. The T cell clone was TCR αß+, CD4+ and appeared to play an important role in the functioning of gut B-lymphocytes. The T cell line had heterogenous population of CD8+ and CD4+ cells, most of which were found to be TCR αß+ and a minor population was TCR γδ+. The lymphokine profile of T cell line showed IFN-γ to be the most abundant lymphokine followed by IL-2 and IL-4. The possible involvement of alternative pathway of activation for T cell clone was also addressed in this study. The splenocytes showed an up-regulation of their CD2 receptor expression on stimulation with the Hsp-24. The pattern of lymphokines released by splenocytes stimulated with the Hsp-24 showed no particular cell type to be responsible for mounting immune response. Thus, there is involvement of both, mucosal and peripheral arm of the immune system.
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Albert MJ, Siddique AK, Islam MS, Faruque SM, Sack RB: 1993 Large outbreak of clinical cholera due to Vibrio cholerae non-01 in Bangladesh Lancet 341: 704
Sack DA, Sack RB, Nair GB, Siddique AK: 2004 Cholera The Lancet 363: 223–233
Chakraborty D, Ghosh A: 2004 Reversal of cyclic AMP of the urea induced inhibition of the synthesis of a catabolic repressible enzyme in V. cholerae J Gen Microbiol 133: 3265–3270
Bradford MM: 1976 A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principles of protein dye binding. Anal Biochem 72: 248–254
Laemmli UK: 1970 Cleavage of ultrastructural protein during the assembly of the head of bacteriophage T4 Nature (London) 227: 680–685
Strahler JR, Kuick R, Hanash SM: 1989 Two dimensional polyacrylamide gel electrophoresis of proteins. In Crighton TE, (ed), Protein Structure: A Practical Approach. IRL Press, Oxford: 65–67
Towbin H, Staachelin T, Gordon J: 1979 Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets: procedure and some application Proc Natl Acad Sci USA 76: 4350–4354
Dass SV, Raja M, Verma RK, Mishra GC: 1995 Characterization of novel costimulatory molecules J Biol Chem 270: 23429–23436
Waite JH, Wang CY: 1976 Spectrophotometric measurement of dodecyl sulfate with basic fuchsin Anal Biochem 70: 279–280
Davies MDJ, Parrot DMV: 1989 Preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine Gut 22: 481–488
Wybran J, Fudenberg HH: 1973 Thymus-derived rosette forming cells N Engl J Med 288: 1072–1073
Jeevanjyot Ghosh A: 1995 Induction of heat shock responses in Vibrio cholerae Microbiology 141: 2101–2109
Neutra MR, Kraehenbuhl JP: 1994 Mucosal immunization via M cells for production of protective secretory IgA antibodies Am J Trop Med 50: 10–13
Richardson SH: 1994 Host susceptibility. In: Wachsmuth IK, Blake PA, Olsvik O (ed), Vibrio cholerae and Cholera: Molecular to Global Perspectives, ASM press, Washington: 273–289
Lemaux PG, Herendeen SL, Bloch PL, Neidhardt FC: 1978 Transient rates of synthesis of individual polypeptides in E. coli following temperature shifts Cell 131: 427–434
Manning PA: 1987 Involvement of cell envelope components with pathogenesis of Vibrio cholerae; targets for cholera vaccine development Vaccine 5: 83–87
Cash RA, Music SI, Libonati JP, Snyder MJ, Wenzel RP, Hornick RB: 1974 Response of man to infection with V. cholerae: clinical, serological and bacteriologic responses to known inoculum J Infect Dis 130: 325–333
Sciortino CV: 1993 Human immune response to the 18 KDa outer membrane antigen of Vibrio cholerae J Med Microbiol 39: 135–140
Abbas AK, Murphy KM, Sher A: 1996 Functional diversity of helper T lymphocytes Nature (London) 383: 787–793
Mowat AMI, Viney JL: 2004 The anatomical and cellular basis of immunity and tolerance in intestine J Pediatr Gastroenterol Nutr 39: 5723–5724
Qadri F, Bhuiyan TR, Dutta KK, Raqib R, Alam MS, Alam NH, Svennerholm AM, Mathan MM: 2004 Acute dehydrating disease caused by Vibrio cholerae serogroups O1 and O139 induce increases in innate cells and inflammatory mediators at the mucosal surface of the gut Gut 53: 62–69
Abreu-Martin MT, Torgon SR: 1996 Regulation of immune responses of the intestinal mucosa Crit Rev Immunol 16: 277–309
Bailey M, Hall L, Bland PW, Stokes CR: 1994 Production of cytokines by lymphocytes from spleen, mesenteric lymph nodes and intestinal lamina propria of pigs Immunology 82: 577–583
Staats HF, Jackson RJ, Marinaro M, Takahashi I, Kiyono H, McGhee JR: 1994 Mucosal immunity to infection with implication for vaccine development Curr Opin Immunol 6: 572–583
Quiding M, Nordstrom I, Kilander A, Anderson G, Hanson LA, Holmgren J, Czerkinsky C: 1991 Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses,gamma interferon productions and evokes local immunological memory J Clin Invest 88: 143–148
Regnault A, Cumano A, Vassali P, Guy Grand D, Kourilsky P: 1994 Oligoclonal repertoire of the CD8 αα and the CD8 αβ TCR αβ murine intestinal intraepithelial T lymphocytes: evidence for random emergence of T cells J Exp Med 180: 1345–1358
Sim GK: 1995 Intraepithelial lymphocytes and the immune system Adv Immunol 58: 297–343
Pirzer UC, Schurman G, Post S, Betzler M, Muer SC Differential responsiveness to CD3 Ti Vs CD2 dependent activation of human intestinal T lymphocytes. Eur J Immunol 20: 2339–2342, 1990
Targan S, Deam RL, Liu M, Wang S, Nel A: 1995 Defination of a lamina propria T cell responsive state. Enhanced cytokine responsiveness of T cells stimulated the CD2 pathway J Immunol 154: 664–675
Svernnerholm AM, Jonson G, Holmgren J 1994 Immunity to Vibrio cholerae infection. In: Wachsmuth IK, Blake PA, Olsvik O (eds), Vibrio cholerae and Cholera. Molecular to Global Perspectives. ASM press. Washington 257–271
Holmgren J: 1991 Mucosal immunity and vaccination FEMS Microbiol Immunol 4: 1–9
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Mazumdar, S., Bhattacharyya, S., Ghosh, S. et al. The role of a heat shock protein from V. cholerae 0139 in the gut immune response. Mol Cell Biochem 297, 9–19 (2007). https://doi.org/10.1007/s11010-006-9316-2
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DOI: https://doi.org/10.1007/s11010-006-9316-2