Skip to main content

Influence of sea buckthorn (Hippophae rhamnoides L.) flavone on dermal wound healing in rats

Abstract

The present investigation was undertaken to determine the efficacy of topical administration of flavone of sea buckthorn (Hippophae rhamnoides L.) on cutaneous wound healing in rats. Four full-thickness excision wounds were created on the back of rat and 1.0% w/v flavone prepared in propylene glycol was applied topically. Control animals received the vehicle alone in an identical manner. The healing of the wound was assessed by the rate of wound contraction, period of epithelialization, hydroxyproline, hexosamine, antioxidants estimation and histopathology of the granulation tissue. The sea buckthorn flavone promoted the wound healing activity as indicated by improved rate of wound contraction, decreased time taken for epithelialization (16.3 days versus 24.8 days in controls) and significant increase in hydroxyproline (26.0%) and hexosamine (30.0%) content. These findings were also confirmed by histopathological examinations. In addition, it was observed that sea buckthorn flavone possesses potent antioxidant properties as evidenced by significant increase in reduced glutathione (55.0%), vitamin C (70.0%) and catalase (20.0%) activities in wound granulation tissue. The flavone treatment also resulted in significant decrease in lipid peroxide levels (39.0%). The results suggest that the sea buckthorn flavone promotes wound healing activity.

This is a preview of subscription content, access via your institution.

References

  1. Clark RAF: Wound repair: Overview and general considerations. In: R.A. Clark and P.M. Henson (eds). The Molecular and Cellular Biology of Wound Repair. Plenum Press, New York, 1996, p 3

  2. Chitra P, Sajithlal GB, Chandrakasan G: Influence of Aloe vera on the glycosamino-glycans in the matrix of healing dermal wounds in rats. J Ethnopharmacol 59: 179–186, 1998

    Article  Google Scholar 

  3. Shukla A, Rasik AM, Jain GK, Shanker R, Kulshrestha DK, Dawan BN: In vito and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. J Ethnopharmacol 65: 1–11, 1999

    Google Scholar 

  4. Udupa AL, Kulkurni DR, Udupa SL: Effect of Tridex procumbens extracts on wound healing. Int J Pharmcognosy 33: 37–40, 1995

    Article  Google Scholar 

  5. Sidhu GS, Singh AK, Thaloor D, Banaudha KK, Paitnaik GK, Srimal RC, Maheshwari RK: Enhancement of wound healing by curcumin in animals. Wound Rep Reg 6: 167–177, 1998

    Article  CAS  Google Scholar 

  6. Rousi A: The genus Hippophae L., a taxonomic study. Annals Bot Fennici 8: 177–227, 1971 (as quoted in ref. 9)

  7. Beveridge T, Li TSC, Oomah BD: Sea buckthorn products: manufacture and composition. J Agric Food Chem 47: 3480–3488, 1999

    PubMed  Article  CAS  Google Scholar 

  8. Suleyman H, Gumustekin K, Taysi S, Keles S, Oztasan N, Aktas O, Altinkaynak K, Timur H, Akcay F, Akar S, Dane S, Gul M: Beneficial effects of Hippophae rhamnoides L. on nicotine induced oxidative stress in rat blood compared with vitamin E. Biol Pharm Bull 25: 1133–1136, 2002

    Article  CAS  Google Scholar 

  9. Geetha S, Sai Ram M, Singh V, Ilavazhagan G, Sawhney RC: Anti-oxidant and immunomodulatory properties of sea buckthorn (Hippophae rhamnoides) an in-vitro study. J Ethnopharmacol 79: 373–378, 2002

    PubMed  Article  CAS  Google Scholar 

  10. Gao ZL, Gu XH, Cheng FT, Jiang FH: Effect of sea buckthorn on liver fibrosis: a clinical study. World J Gastroenterol 9: 1615–1617, 2003

    PubMed  Google Scholar 

  11. Gupta A, Kumar R, Pal K, Banerjee PK, Sawhney RC: A preclinical study of the effects of sea buckthorn (Hippophae rhamnoides L.) leaf extract on cutaneous wound healing in albino rats. Int J Low Extrem Wounds 4: 88–92, 2005

    PubMed  Article  Google Scholar 

  12. Suleyman H, Buyukokuroglu ME, Koruk M, Akcay F, Kiziltunc A, Geptiremen A: The effects of Hippophae rhamnoides L. extract on ethanol-induced gastric lesion and gastric tissue glutathione level in rats: a comparative study with melatonin and omeprazole. Indian J Pharmacol 33: 77–81, 2001

    Google Scholar 

  13. Xing J, Yang B, Dong Y, Wang B, Wang J, Kallio HP: Effects of sea buckthorn (Hippophae rhamnoides L.) seed and pulp oils on experimental models of gastric ulcer in rats. Fitoterapia 73: 644–650, 2002

    PubMed  Article  CAS  Google Scholar 

  14. Cheng J, Kondo K, Suzuki Y, Ikeda Y, Meng X, Umemura K: Inhibitory effects of total flavones of Hippophae rhamnoides L. on thrombosis in mouse femoral artery and in-vitro platelet aggregation. Life Sci 72: 2263–2271, 2003

    PubMed  Article  CAS  Google Scholar 

  15. Eccleston C, Baoru Y, Tahvonen R, Kallio H, Rimbach GH, Minihane AM: Effects of an antioxidant-rich juice (sea buckthorn) on risk factors for coronary heart disease in humans. J Nutr Biochem 13: 346–354, 2002

    PubMed  Article  CAS  Google Scholar 

  16. Singleton VL, Rossi JA: Colorimetry of total phenolics with phosphomolybidic phosphotungstic acid reagents. Am J Enol Viticult 16: 144–158, 1965

    CAS  Google Scholar 

  17. Gupta A, Jain GK, Raghubir R: A time course study for the development of an immunocompromised wound model, using hydrocortisone. J Pharmocol Toxicol 41: 183–187, 1999

    Article  CAS  Google Scholar 

  18. Buffoni F, Banchelli G, Cambe S, Ignesti G, Pirisino R, Raimond L, Vannelli G: Skin wound healing: some biochemical parameters in Guinea-pig. J Pharm Pharmacol 45: 784–790, 1993

    PubMed  CAS  Google Scholar 

  19. Woessner JF Jr: The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acids. Arch Biochem Biophys 93: 440–447, 1961

    PubMed  Article  CAS  Google Scholar 

  20. Elson LA, Morgan WTJ: A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J 27: 1824–1828, 1933

    PubMed  CAS  Google Scholar 

  21. Gupta A, Singh RL, Raghubir R: Antioxidant status during cutaneous wound healing in immunocompromised rats. Mol Cellular Biochem 241: 1–7, 2002

    Article  CAS  Google Scholar 

  22. Beutler E, Duron O, Kelly BM: Improved method for the determination of blood glutathione. J Lab Clin Med 61: 882–888, 1963

    PubMed  CAS  Google Scholar 

  23. Marklund A, Marklund G: Involvement of the superoxide anion radical in the auto-oxidation of pyrogallol and convenient assay for superoxide dismutase. Eur J Biochem 47: 469–474, 1974

    PubMed  Article  CAS  Google Scholar 

  24. Aebi H: Catalase. In: H.U. Bergmeyer (ed). Methods of Enzymatic Analysis. Academic Press, New York, 1984, pp 673–684

  25. Rae JH: Chemical determination of ascorbic acid, dehydroascorbic acid and diketogluconic acids. In: Q. Glick (ed). Methods of Biochemical Analysis. Interscience Publishers, New York, 1984, pp 115–139

  26. Ohkawa H, Ohishi N, Yogi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351–358, 1979

    PubMed  Article  CAS  Google Scholar 

  27. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ: Protein measurement with Folin phenol reagent. J Biol Chem 193: 265–275, 1951

    PubMed  CAS  Google Scholar 

  28. Yuzhen Z, Fuheng W: Sea buckthorn flavonoids and their medical value. Hippophae 10: 39–41, 1997

    Google Scholar 

  29. Khanna S, Venojarvi M, Roy S, Sharma N, Trikha P, Bagchi D, Bagchi M, Sen CK: Dermal wound healing properties of redox-active grape seed proanthocyanidins. Free Rad Biol Med 33: 1089–1096, 2002

    PubMed  Article  CAS  Google Scholar 

  30. Kapoor M, Howard R, Hall I, Appleton I: Effects of epicatechin gallate on wound healing and scar formation in a full thickness incisional wound healing model in rats. Am J Pathol 165: 299–307, 2004

    PubMed  CAS  Google Scholar 

  31. Gomathi K, Gopinath D, Rafiuddin AM, Jayakumar R: Quercetin incorporated collagen matrices for dermal wound healing processes in rats. Biomaterials 24: 2767–72, 2003

    PubMed  Article  CAS  Google Scholar 

  32. Selvam R, Sumramanian L, Gayathri R, Angayarkanni N: The anti-oxidant activity of turmeric (Curcuma longa). J Ethanopharmacol 47: 59–67, 1995

    Article  CAS  Google Scholar 

  33. Pascoe GA, Fariss MW, Olafsdottir K, Reed DJ: A role of vitamin E in protection against cell injury: Maintenance of intracellular glutathione precursor and biosynthesis. Eur J Biochem 166: 241–247, 1987

    PubMed  Article  CAS  Google Scholar 

  34. De-Groot H, Rauen U: Tissue injury by reactive oxygen species and the protective effects of flavonoids. Fundam Clin Pharmacol 12: 249–255, 1998

    PubMed  CAS  Article  Google Scholar 

  35. Kivirikko KI, Prockop DJ: Enzymatic hydroxylation of proline and lysine in protocollagen. Proc Natl Acad Sci USA 57: 782–789, 1967

    PubMed  Article  CAS  Google Scholar 

  36. Chojkier M, Houglum K, Herruzo JS, Brenner DA: Stimulation of collagen gene expression by ascorbic acid in cultured human fibroblasts: A role for lipid peroxidation. J Biol Chem 264: 16957–16962, 1989

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Asheesh Gupta.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Gupta, A., Kumar, R., Pal, K. et al. Influence of sea buckthorn (Hippophae rhamnoides L.) flavone on dermal wound healing in rats. Mol Cell Biochem 290, 193 (2006). https://doi.org/10.1007/s11010-006-9187-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11010-006-9187-6

Keywords

  • sea buckthorn flavone
  • wound healing
  • collagen
  • antioxidant