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Age- and cell cycle-dependent changes in EPC-1/PEDF promoter activity in human diploid fibroblast-like (HDF) cells

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Abstract

The changes in gene expression during senescence are very interesting. Early population doubling cDNA-1 (EPC-1, also known as pigment epithelial derived factor, PEDF) is one of the genes whose expression decreases dramatically during cellular aging. We examined whether or not EPC-1/PEDF promoter activity was affected by the cellular ageing using human diploid lung fibroblast cells in culture. Here we showed the promoter/enhancer region of EPC-1/PEDF existed at more than 1760 bp upstream from the transcriptional initiation site of the gene, and was regulated by both aging and cell cycle. These findings suggest that the expression of the EPC-1/PEDF gene is, at least in part, regulated transcriptionally in the cells. The analysis of the promoter region of the EPC-1/PEDF gene in this paper suggests the age- and cell cycle-dependent expression of specific transcriptional factor(s).

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Authors and Affiliations

  1. Department of Cellular Physiological Chemistry, Graduated School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Toshihiko Kojima, Ken-ichi Nakahama & Ikuo Morita

  2. Department of Gerodontology, Graduated School, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan

    Toshihiko Kojima & Hiroshi Uematsu

  3. Department of Biology, Tokyo Metropolitan Institute of Gerontology, Sakae-cho, Itabashi-ku, Tokyo, Japan

    Kiyotaka Yamamoto

  4. Center of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone, Japan

    Ikuo Morita

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  1. Toshihiko Kojima
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  2. Ken-ichi Nakahama
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  3. Kiyotaka Yamamoto
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  4. Hiroshi Uematsu
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  5. Ikuo Morita
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Correspondence to Ken-ichi Nakahama.

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Kojima, T., Nakahama, Ki., Yamamoto, K. et al. Age- and cell cycle-dependent changes in EPC-1/PEDF promoter activity in human diploid fibroblast-like (HDF) cells. Mol Cell Biochem 293, 63–69 (2006). https://doi.org/10.1007/s11010-006-2680-0

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  • DOI: https://doi.org/10.1007/s11010-006-2680-0

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