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Effects of phosphates on the expression of tissue-nonspecific alkaline phosphatase gene and phosphate-regulating genes in short-term cultures of human osteosarcoma cell lines

Abstract

We studied the effects of phosphates on the expression of the human tissue-nonspecific alkaline phosphatase (TNSALP) gene and phosphate-regulating genes in short-term cultures of human osteoblastic osteosarcoma cell lines. When human osteosarcoma cell lines, SaOS-2, MG-63, and U2OS were cultured with 10 mM inorganic sodium dihydrogenphosphate, 10 mM β-glycerophosphate, 250 μM pyridoxal phosphate, or 100 μM inorganic pyrophosphate, enzymatic activity of alkaline phosphatase began to increase at 72 h after addition of sodium dihydrogenphosphate and β-glycerophosphate in SaOS-2 cells. Pyridoxal phosphate and pyrophosphate did not induce alkaline phosphatase activity. U2OS cells slightly reacted to β-glycerophosphate, but MG-63 cells did not react on exposure to phosphates. In SaOS-2 cells, TNSALP mRNA measured by real-time RT-PCR reached a peak level at 72 h after the addition of β-glycerophosphate. PHEX and MEPE mRNAs were also induced by β-glycerophosphate. These results suggest that TNSALP, PHEX and MEPE were concordantly induced by β-glycerophosphate on mineralisation.

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Correspondence to Hideo Orimo.

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Orimo, H., Shimada, T. Effects of phosphates on the expression of tissue-nonspecific alkaline phosphatase gene and phosphate-regulating genes in short-term cultures of human osteosarcoma cell lines. Mol Cell Biochem 282, 101–108 (2006). https://doi.org/10.1007/s11010-006-1520-6

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  • DOI: https://doi.org/10.1007/s11010-006-1520-6

Key words

  • expression
  • MEPE
  • osteosarcoma cell lines
  • PHEX
  • phosphates
  • tissue-nonspecific alkaline phosphatase