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Effects of phosphates on the expression of tissue-nonspecific alkaline phosphatase gene and phosphate-regulating genes in short-term cultures of human osteosarcoma cell lines

Molecular and Cellular Biochemistry volume 282, pages 101–108 (2006)Cite this article

Abstract

We studied the effects of phosphates on the expression of the human tissue-nonspecific alkaline phosphatase (TNSALP) gene and phosphate-regulating genes in short-term cultures of human osteoblastic osteosarcoma cell lines. When human osteosarcoma cell lines, SaOS-2, MG-63, and U2OS were cultured with 10 mM inorganic sodium dihydrogenphosphate, 10 mM β-glycerophosphate, 250 μM pyridoxal phosphate, or 100 μM inorganic pyrophosphate, enzymatic activity of alkaline phosphatase began to increase at 72 h after addition of sodium dihydrogenphosphate and β-glycerophosphate in SaOS-2 cells. Pyridoxal phosphate and pyrophosphate did not induce alkaline phosphatase activity. U2OS cells slightly reacted to β-glycerophosphate, but MG-63 cells did not react on exposure to phosphates. In SaOS-2 cells, TNSALP mRNA measured by real-time RT-PCR reached a peak level at 72 h after the addition of β-glycerophosphate. PHEX and MEPE mRNAs were also induced by β-glycerophosphate. These results suggest that TNSALP, PHEX and MEPE were concordantly induced by β-glycerophosphate on mineralisation.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan

    Hideo Orimo & Takashi Shimada

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  1. Hideo Orimo
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  2. Takashi Shimada
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Correspondence to Hideo Orimo.

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Orimo, H., Shimada, T. Effects of phosphates on the expression of tissue-nonspecific alkaline phosphatase gene and phosphate-regulating genes in short-term cultures of human osteosarcoma cell lines. Mol Cell Biochem 282, 101–108 (2006). https://doi.org/10.1007/s11010-006-1520-6

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  • DOI: https://doi.org/10.1007/s11010-006-1520-6

Key words

  • expression
  • MEPE
  • osteosarcoma cell lines
  • PHEX
  • phosphates
  • tissue-nonspecific alkaline phosphatase