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Human placental protein/peptides stimulate melanin synthesis by enhancing tyrosinase gene expression

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Abstract

Placental protein/peptides as biological response modifier are well documented, but not much known about melanogenesis. We possibly for the first time, demonstrated melanogenesis in B16F10 mouse melanoma by a placental protein/peptide fraction (PPPF) prepared from a hydroalcoholic extract of fresh term human placenta. This study described the effect of PPPF on the induction of tyrosinase; the key enzyme of melanogenesis to investigate the basis of PPPF induced pigmentation in primary melanocyte and B16F10 melanoma. Tyrosinase induction by PPPF in B16F10 cells was found dose- and time dependent at the level of activity. Tyrosinase, at the level of transcription and protein expression when assessed by RT-PCR and Western blot analyses found to have considerable induction over untreated control. PPPF led to enhanced activation of tyrosinase promoter resulting higher transcription thus substantiating the role of PPPF as a stimulator of melanogenesis. Actinomycin D, the transcriptional inhibitor of protein synthesis, blocked the stimulatory action of PPPF since the induction of tyrosinase and melanin was markedly reduced in presence of this inhibitor. Thus the results suggested that PPPF mediated increase in tyrosinase expression occurred through transcriptional upregulation to stimulate melanogenesis in B16F10 cells and in primary melanocyte also. (Mol Cell Biochem xxx: 1–10, 2004)

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Authors and Affiliations

  1. Department of Cellular Biochemistry, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, 700032, Jadavpur, Kolkata, India

    Chinmoy Sarkar, Suman Kumar Singh, Bidisha Saha, Rabindranath Bera, Jagnyeswar Ratha & Ranjan Bhadra

  2. Department of Dermatology and STD, Medical College and Hospital, Calcutta, Kolkata, India

    Pijush Kanti Datta

  3. Biomedical Research Division, The University of Texas Health Center at Tyler, Tyler, Texas, USA

    Samir Kumar Mandal

Authors
  1. Chinmoy Sarkar
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  2. Suman Kumar Singh
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  3. Samir Kumar Mandal
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  4. Bidisha Saha
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  5. Rabindranath Bera
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  7. Pijush Kanti Datta
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Correspondence to Ranjan Bhadra.

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Sarkar, C., Singh, S.K., Mandal, S.K. et al. Human placental protein/peptides stimulate melanin synthesis by enhancing tyrosinase gene expression. Mol Cell Biochem 285, 133–142 (2006). https://doi.org/10.1007/s11010-005-9069-3

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  • DOI: https://doi.org/10.1007/s11010-005-9069-3

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