Molecular and Cellular Biochemistry

, Volume 285, Issue 1–2, pp 121–131 | Cite as

Protective potentials of a potentized homeopathic drug, Lycopodium-30, in ameliorating azo dye induced hepatocarcinogenesis in mice

  • Surajit Pathak
  • Jayanta Kumar Das
  • Surjyo Jyoti Biswas
  • Anisur Rahman Khuda-Bukhsh


The protective potentials of a potentized homeopathic drug, Lycopodium-30, prepared from extract of spores of a plant, Lyocopodium clavatum (Fam: Lycopodiaceae) and used as a remedy for various liver ailments, have been tested in mice chronically fed p-dimethyl amino azo benzene (p-DAB) – an initiator, and phenobarbital (PB) – a promoter of hepatic cancer, by using some cytogenetic endpoints like chromosome aberrations (CA), micronuclei (MN), mitotic index (MI) and sperm head abnormality (SHA), and toxicity biomarkers like acid and alkaline phosphatases (AcP and AlkP, respectively), alanine and aspartate amino transferases (ALT and AST, respectively) and lipid peroxidation (LPO) and reduced glutathione (GSH) activities. The effects of chronic treatment of the carcinogens were assessed at different intervals of fixation, namely, at day 7, 15, 30, 60, 90 and day 120, and compared with that of mice fed conjointly with the carcinogens and the homeopathic remedy. Both the assay systems indicated considerable protective potentials of the homeopathic remedy against p-DAB induced hepatocarcinogenesis in mice.

Key words

p-DAB hepatocarcinogenesis toxicity biomarkers Lycopodium-30 hapatoprotection 



deoxyribonucleic acid


p-dimethyl amino azo benzene




chromosome aberrations




mitotic index


sperm head abnormality


acid phosphatases


alkaline phosphatases


alanine amino transferases


aspartate amino transferases


lipid peroxidation


reduced glutathione


reactive oxygen species


5,5′-Dithiobis (2 nitro benzoic acid)


Aminoazo benzene


Mono aminoazo benzene


Complimentary & Alternative Medicine




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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Surajit Pathak
    • 1
  • Jayanta Kumar Das
    • 1
  • Surjyo Jyoti Biswas
    • 1
  • Anisur Rahman Khuda-Bukhsh
    • 1
  1. 1.Department of Zoology, Cytogenetics and Molecular Biology LaboratoryUniversity of KalyaniKalyaniIndia

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