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Smoke treatment triggers the release of a novel DNA damaging factor by lymphocytes

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Organic fuel smoke is a hazardous agent, which pushes the cells towards“prooxidant state'', leading to 4,46,400 strand breaks/cell/day as against 47,000 strand breaks/cell/day produced by constitutive oxygen radicals. This prooxidants scenario switches on a plethora of intercellular events. Here we report a novel DNA damaging factor released by lymphocytes, upon treatment with smoke condensate. Human lymphocytes, when exposed to cow dung cake smoke condensate, were found to release a low molecular weight factor into the media at 20 min of exposure. The conditioned media, displayed a propensity of inducing DNA damage in fresh, normal lymphocytes, which were not exposed to any damaging agent. The above DNA damaging effect of the conditioned media was not due to any residual presence of Polycyclic Aromatic Hydrocarbons, which were present in the smoke. The release of this factor was in correlation with the DNA damaging event, taking place in the cells. This secondary DNA damaging factor had a molecular weight less than 5 kd. The factor had the cell death inducing propensity when allowed to act on lymphocytes

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Abbreviations

CAT:

catalase

COX:

cyclooxygenase

CSC:

cigarette smoke condensate

DMSO:

dimethyl sulfoxide

EGTA:

ethylene glycol (bis ethyl ether) tetra acetic acid

FADU:

fluorimetric analysis of DNA unwinding

HBSS:

Hank's balanced salt solution

Kd:

kilo daltons

PAH:

polycyclic aromatic hydrocarbons

PBS:

phosphate buffered saline

ROS:

reactive oxygen species

SOD:

superoxide dismutase

TLCK:

tosyl lysyl chloromethyl ketone

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Authors and Affiliations

  1. Indian Institute of Science, Bangalore, 12, Karnataka, India

    Raghavendra Pralhada Rao

  2. Adichunchanagiri Cancer Research Institute, B.G. Nagara, 571 448, Bangalore, Karnataka, India

    S. S. Thammanna Gowda & Srinivas Leela

  3. Adichunchanagiri Biotechnology and Cancer Research Institute, B.G. Nagara, Nagamangala Talluk, Mandya District, 571 448, Bangalore, Karnataka, India

    Srinivas Leela

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  1. Raghavendra Pralhada Rao
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  2. S. S. Thammanna Gowda
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  3. Srinivas Leela
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Correspondence to Srinivas Leela.

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Rao, R.P., Gowda, S.S.T. & Leela, S. Smoke treatment triggers the release of a novel DNA damaging factor by lymphocytes. Mol Cell Biochem 284, 73–79 (2006). https://doi.org/10.1007/s11010-005-9015-4

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  • DOI: https://doi.org/10.1007/s11010-005-9015-4

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