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Sensitivity of mitochondria isolated from liver and kidney of rat and bovine to lipid peroxidation: A comparative study of light emission and fatty acid profiles

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Abstract

Much work has been carried out on non-enzymatic–induced lipid peroxidation of mitochondria obtained from different tissues of monogastric animals, but little information is available about this process in poligastric animals. Studies were carried out to determine the sensitivity of mitochondria isolated from liver and kidney of rat and bovine to lipid peroxidation (ascorbate-Fe2+ dependent) by comparison of light emission and fatty acid profiles. Mitochondria from both species were susceptible to lipid peroxidation. Measurements of chemiluminescence indicate that the lipid peroxidation process was more effective in mitochondria from rat liver than in the organelle obtained from bovine, whereas changes were not observed in mitochondria from rat and bovine kidney. The fatty acid composition of total lipids isolated from liver and kidney mitochondria of both species was substantially modified when subjected to non-enzymatic lipid peroxidation with a decrease of arachidonic and docosahexaenoic acids. The polyunsaturated fatty acid (PUFA) composition was higher in mitochondria obtained from rat liver (43.11± 4.16) than in bovine (15.78 ± 0.76). As a consequence, the unsaturation index (UI), was higher in mitochondria of rat liver than in bovine. Nevertheless, the PUFA composition of kidney mitochondria from both species was similar; therefore, statistically significant differences in the UI were not observed. The results suggest that mainly the PUFAs present in hepatic and kidney mitochondria were sensitive to oxidative damage. The lipid peroxidation process was more effective in rat liver mitochondria than in bovine. (Mol Cell Biochem xxx: 77–82, 2005)

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References

  1. Sies H: Strategies of antioxidant defense. Eur J Biochem 215: 213–219, 1993

    Article  PubMed  CAS  Google Scholar 

  2. Pamplona R, Portero-Otin M, Riba D, Ruiz C, Prat J, Bellmunt MJ, Barja G: Mitochondrial membrane peroxidizability index is inversely related to maximum life span in mammals. J Lipid Res 39: 1989–1994, 1998

    PubMed  CAS  Google Scholar 

  3. Rosa D, Catalá A: Fatty acid profiles and non enzymatic lipid peroxidation of microsomes and mitochondria from bovine liver, kidney, lung and heart. Arch Phisiol Biochem 106: 33–37, 1998

    CAS  Google Scholar 

  4. Catalá A, Cerruti A: Non enzymatic peroxidation of lipids isolated from rat liver microsomes, mitochondria and nuclei. Int J Biochem Cell Biol 29: 541–546, 1997

    PubMed  Google Scholar 

  5. Dix TA, Aikens J: Mechanism and biological relevance of lipid peroxidation initiation. Chem Res Toxicol 6: 2–18, 1993

    Article  PubMed  CAS  Google Scholar 

  6. Vladimirov Yu A, Olenev VI, Suslova TB, Cheremisina ZP: Lipid peroxidation in mitochondrial membrane. Adv Lipid Res 17: 173–249, 1980

    Google Scholar 

  7. Minotti G, Aust SD: Redox cycling of iron and lipid peroxidation. Lipids 27: 219–226, 1992

    PubMed  CAS  Google Scholar 

  8. Zanetti R, Catalá A: Changes in n-6 and n-3 polyunsaturated fatty acids during lipid peroxidation of mitochondria obtained from rat liver and several brain regions: effect of α-tocopherol. Prostaglandins Leukot Essent Fatty Acids 62: 379–385, 2000

    Article  PubMed  CAS  Google Scholar 

  9. Ozgova S, Hermanek J, Gut I: Different antioxidant effects of polyphenols on lipid peroxidation and hydroxyl radicals in the NADPH, Fe-ascorbate and Fe microsomal systems. Biochem Pharmacol 66: 1127–37, 2003

    PubMed  CAS  Google Scholar 

  10. Dmitriev LF: Activity of key enzymes in microsomal and mitochondrial membranes depends on the redox reactions involving lipid radicals. Membr Cell Biol 14: 649–62, 2001

    PubMed  CAS  Google Scholar 

  11. Hsieh RJ, Kinsella JE: Oxidation of polyunsaturated fatty acids: mechanism, products, and inhibition with emphasis on fish. Adv Food Nutr Res 33: 233–241, 1989

    PubMed  CAS  Google Scholar 

  12. Halliwell B, Gutteridge JMC: Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol 186: 1–85, 1990

    PubMed  CAS  Google Scholar 

  13. Palmer S: Antioxidant vitamins and cancer risk. Nutrition 10: 433–434, 1994

    Google Scholar 

  14. Aruoma OI: Nutrition and health aspects of free radicals and antioxidants. Food Chem Toxicol 32: 671–683, 1994

    PubMed  CAS  Google Scholar 

  15. Bonorden WR, Pariza MW: Antioxidant nutrients and protection from free radicals. In: F.N. Kotsonics (ed). Nutritional Toxicology, Raven Press, New York, 1994, pp 19–45

  16. Llanillo M, Sanchez Yague J, Checa A, Martín-Valmaseda EM, Felipe A: Phospholipid and fatty acid composition in stored sheep erythrocytes of different densities. Exp Hematol 23: 258–264, 1995

    PubMed  CAS  Google Scholar 

  17. Schneider WC, Hogeboom HG: Intracellular distribution of enzymes. Further studies and distribution of cytochrome c in rat liver homogenates. J Biol Chem 178: 123–128, 1950

    Google Scholar 

  18. Wright JR, Rumbaugh RC, Colby HD, Miles PR: The relationship between chemiluminescence and lipid peroxidation in rat hepatic microsomes. Arch Biochem Biophys 192: 344–351, 1979

    PubMed  CAS  Google Scholar 

  19. Tadolini B, Cabrini L, Menna C, Pinna GG, Hakim G: Iron [III] stimulation of lipid hydroperoxide-dependent lipid peroxidation. Free Rad Res 27: 563–576, 1997

    Article  CAS  Google Scholar 

  20. Folch J, Lees N, Sloane Stanley GA: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509, 1957

    PubMed  CAS  Google Scholar 

  21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951

    PubMed  CAS  Google Scholar 

  22. Gutierrez AM, Reboredo GR, Catala A: Fatty acid profiles and lipid peroxidation of microsomes and mitochondria from liver, heart and brain of Cairina Moschata. Int J Biochem Cell Biol 34: 605–612, 2002

    PubMed  CAS  Google Scholar 

  23. Iglesias BF, Catalá A: Rat, caprine, equine and bovine erythrocyte ghosts exposed to t-butyl hydroperoxide as a model to study lipid peroxidation using a chemiluminescence assay. Res Vet Sci 79: 19–27, 2005

    Article  PubMed  CAS  Google Scholar 

  24. Pamplona R, Prat J, Cadenas S, Rojas C, Pérez-Campo R, López Torres M, Barja G: Low fatty acid unsaturation protects against lipid peroxidation in liver mitochondria from long-lived species: the pigeon and human case. Mech Age Dev 86: 53–66, 1996

    Article  CAS  Google Scholar 

  25. Gutierrez AM, Reboredo GR, Arcemis CJ, Catalá A: Non-enzymatic lipid peroxidation of microsomes and mitochondria isolated from liver and heart of pigeon and rat. Int J Biochem Cell Biol 32: 73–79, 2000

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296, B1900 AVW, La Plata, Argentina

    Mariana Gavazza, Mónica Marmunti & Angel Catalá

  2. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, CIC. Casilla de Correo 16, Sucursal 4, 1900, La Plata, Argentina

    Angel Catalá

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  1. Mariana Gavazza
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  2. Mónica Marmunti
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  3. Angel Catalá
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Correspondence to Angel Catalá.

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Member of Carrera del Investigador Científico, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)

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Gavazza, M., Marmunti, M. & Catalá, A. Sensitivity of mitochondria isolated from liver and kidney of rat and bovine to lipid peroxidation: A comparative study of light emission and fatty acid profiles. Mol Cell Biochem 280, 77–82 (2005). https://doi.org/10.1007/s11010-005-8233-0

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