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The progressive effects of a fat enriched diet on ventricular myocyte contraction and intracellular Ca2+ in the C57BL/6J mouse

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Abstract

The C57BL/6J mouse has a genetic susceptibility to develop diabetes when fed with a high-fat, high-sucrose diet. The general characteristics of diet-induced diabetes in this model include progressive development of hyperinsulinaemia, hyperglycaemia, insulin resistance and obesity, features that are frequently observed in the clinical setting. This study investigated the progressive effects of a fat enriched (FE) diet on contraction and intracellular Ca2+ in ventricular myocytes from the C57BL/6J mouse. The characteristics of the mice fed with the FE diet compared to mice receiving control diet included progressive increase in the rate of body weight gain, increased fasting blood glucose and time-dependent differences in the disposal of blood glucose after a glucose challenge. The ultrastructure of cardiac myocytes and associated capillaries did not show any gross morphological alteration after 27 weeks of FE diet compared to controls.

At 5 months the resting cell length (RCL) and the kinetics of shortening were not significantly altered in ventricular myocytes from mice receiving the FE diet compared to age-matched controls. At 5 and at 7 months the amplitude of shortening was increased in myocytes receiving the FED diet compared to controls. At 7 months the time to half (THALF) relaxation of myocyte contraction was shortened in myocytes from mice receiving the FE diet compared to controls. Mean THALF relaxation in myocytes from mice fed the FE diet was 32.0 ± 1.4 ms (n = 23) compared to 40.2 ± 2.0 ms (n = 27) in controls. Neither resting intracellular Ca2+ nor the kinetics or amplitude of the Ca2+ transient were altered by FE diet. Differences in myofilament sensitivity to Ca2+ might underlie the changes in contractility.

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References

  1. Amos AF, McCarty DJ, Zimmet P: The rising global burden of diabetes and its complications: Estimates and projections to the year 2010. Diab Med 14: S7–S85, 1997

    Article  Google Scholar 

  2. Dhalla NS, Pierce GN, Innes IR, Beamish RE: Pathogenesis of cardiac dysfunction in diabetes mellitus. Can J Cardiol 1: 263–281, 1985

    CAS  PubMed  Google Scholar 

  3. Choi KM, Zhong Y, Hoit BD, Grupp IL, Hahn H, Dilly KW, Guatimosim S, Lederer WJ, Matlib MA: Defective intracellular Ca(2+) signaling contributes to cardiomyopathy in Type 1 diabetic rats. Am J Physiol 283: H1398–H1408, 2002

    CAS  Google Scholar 

  4. Ren J, Davidoff AJ: Diabetes rapidly induces contractile dysfunctions in isolated ventricular myocytes. Am J Physiol 41: H148–H158, 1997

    Google Scholar 

  5. Yu Z, Tibbits GF, McNeill JH: Cellular functions of diabetic cardiomyocytes: Contractility, rapid- cooling contracture, and ryanodine binding. Am J Physiol 266: H2082–H2089, 1994

    CAS  PubMed  Google Scholar 

  6. Howarth FC, Qureshi MA, Bracken NK, Winlow W, Singh J: Time-dependent effects of streptozotocin-induced diabetes on contraction of ventricular myocytes from rat heart. Emirates Med J 19: 35–41, 2001

    Google Scholar 

  7. Okayama H, Hamada M, Hiwada K: Contractile dysfunction in the diabetic-rat heart is an intrinsic abnormality of the cardiac myocyte. Clin Sci (Colch) 86: 257–262, 1994

    CAS  Google Scholar 

  8. Chattou S, Diacono J, Feuvray D: Decrease in sodium–calcium exchange and calcium currents in diabetic rat ventricular myocytes. Acta Physiol Scand 166: 137–144, 1999

    Article  CAS  PubMed  Google Scholar 

  9. Bracken NK, Woodall A, Howarth FC, Singh J: Voltage-dependence of contraction in streptozotocin-induced diabetic ventricular myocytes. Mol Cel Biochem 261: 233–243, 2004

    Google Scholar 

  10. Surwit RS, Seldin MF, Kuhn CM, Cochrane C, Feinglos MN: Control of expression of insulin resistance and hyperglycemia by different genetic factors in diabetic C57BL/6J mice. Diabetes 40: 82–87, 1991

    CAS  PubMed  Google Scholar 

  11. Black BL, Croom J, Eisen EJ, Petro AE, Edwards CL, Surwit RS: Differential effects of fat and sucrose on body composition in A/J and C57BL/6 mice. Metabolism 47: 1354–1359, 1998

    Article  CAS  PubMed  Google Scholar 

  12. Howarth FC, Qureshi MA: Characterisation of ventricular myocyte shortening after administration of streptozotocin (STZ) to neonatal rats. Arch Physiol Biochem 109: 200–205, 2001

    Article  CAS  PubMed  Google Scholar 

  13. McDowell EM, Trump BF: Histologic fixatives suitable for diagnostic light and electron microscopy. Arch Pathol Lab Med 100: 405–414, 1976

    CAS  PubMed  Google Scholar 

  14. Draper CE, Adeghate E, Lawrence PA, Pallot DJ, Garner A, Singh J: Age-related changes in morphology and secretory responses of male rat lacrimal gland. J Auton Nerv Syst 69:173–183, 1998

    Article  CAS  PubMed  Google Scholar 

  15. Reynolds ES: The use of lead citrate at high pH as an electron-opaque staining in electron microscopy. J Cell Biol 17: 208–212, 1963

    Article  CAS  PubMed  Google Scholar 

  16. Howarth FC, Qureshi MA, White E: Effects of hyperosmotic shrinking on ventricular myocyte shortening and intracellular Ca(2+) in streptozotocin-induced diabetic rats. Pflügers Arch 444: 446–451, 2002

    Article  CAS  PubMed  Google Scholar 

  17. White E, Boyett MR, Orchard CH: The effects of mechanical loading and changes of length on single guinea-pig ventricular myocytes. J Physiol 482: 93–107, 1995

    CAS  PubMed  Google Scholar 

  18. Martineau LC, Chadan SG, Parkhouse WS: Age-associated alterations in cardiac and skeletal muscle glucose transporters, insulin and IGF-1 receptors, and PI3-kinase protein contents in the C57BL/6 mouse. Mech Ageing Dev 106: 217–232, 1999

    Article  CAS  PubMed  Google Scholar 

  19. Yang B, Larson DF, Watson R: Age-related left ventricular function in the mouse: Analysis based on in vivo pressure-volume relationships. Am J Physiol 277: H1906–H1913, 1999

    CAS  PubMed  Google Scholar 

  20. Schaffer SW, Tan BH, Wilson GL: Development of a cardiomyopathy in a model of noninsulin-dependent diabetes. Am J Physiol 248: H179–H185, 1985

    CAS  PubMed  Google Scholar 

  21. Ren J, Porter JE, Wold LE, Aberle NS, Muralikrishnan D, Haselton JR: Depressed contractile function and adrenergic responsiveness of cardiac myocytes in an experimental model of Parkinson disease, the MPTP-treated mouse. Neurobiol Aging 25: 131–138, 2004

    Article  CAS  PubMed  Google Scholar 

  22. Crozatier B, Badoual T, Boehm E, Ennezat PV, Guenoun T, Su J, Veksler V, Hittinger L, Ventura-Clapier R: Role of creatine kinase in cardiac excitation-contraction coupling: Studies in creatine kinase-deficient mice. FASEB J 16: 653–660, 2002

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates

    F. C. Howarth & M. A. Qureshi

  2. Department of Anatomy, Faculty of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates

    A. J. Gbewonyo, S. Tariq & E. Adeghate

  3. Department of Physiology, Faculty of Medicine & Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, United Arab Emirates

    F. C. Howarth

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  1. F. C. Howarth
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  2. M. A. Qureshi
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  3. A. J. Gbewonyo
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  4. S. Tariq
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  5. E. Adeghate
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Correspondence to F. C. Howarth.

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Howarth, F.C., Qureshi, M.A., Gbewonyo, A.J. et al. The progressive effects of a fat enriched diet on ventricular myocyte contraction and intracellular Ca2+ in the C57BL/6J mouse. Mol Cell Biochem 273, 87–95 (2005). https://doi.org/10.1007/s11010-005-7758-6

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  • DOI: https://doi.org/10.1007/s11010-005-7758-6

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