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Decreased 5-HT2C receptor binding in the cerebral cortex and brain stem during pancreatic regeneration in rats

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Abstract

The purpose of this study was to investigate the role of central 5-HT2C receptor binding in rat model of pancreatic regeneration using 60–70% pancreatectomy. The 5-HT and 5-HT2C receptor kinetics were studied in cerebral cortex and brain stem of sham operated, 72 h pancreatectomised and 7 days pancreatectomised rats. Scatchard analysis with [3H] mesulergine in cerebral cortex showed a significant decrease (p < 0.05) in maximal binding (Bmax) without any change in Kd in 72 h pancreatectomised rats compared with sham. The decreased Bmax reversed to sham level by 7 days after pancreatectomy. In brain stem, Scatchard analysis showed a significant decrease (p < 0.01) in Bmax with a significant increase (p < 0.01) in Kd. Competition analysis in brain stem showed a shift in affinity towards a low affinity. These parameters were reversed to sham level by 7 days after pancreatectomy. Thus the results suggest that 5-HT through the 5-HT2C receptor in the brain has a functional regulatory role in the pancreatic regeneration.

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Authors and Affiliations

  1. Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin, India

    Valiya Veettil Mohanan, Finla Chathu & Cheramadathikudyil S. Paulose

  2. Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin, 682 022, Kerala, India

    Cheramadathikudyil S. Paulose

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  1. Valiya Veettil Mohanan
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  2. Finla Chathu
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Correspondence to Cheramadathikudyil S. Paulose.

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Mohanan, V.V., Chathu, F. & Paulose, C.S. Decreased 5-HT2C receptor binding in the cerebral cortex and brain stem during pancreatic regeneration in rats. Mol Cell Biochem 272, 165–170 (2005). https://doi.org/10.1007/s11010-005-7030-0

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  • DOI: https://doi.org/10.1007/s11010-005-7030-0

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