Abstract
Purpose: Evidence indicates that free radicals are etiological factors in obstructive bladder disease. However, it is not clear which species of reactive oxygen or nitrogen species mediate the damage. The current studies were designed to determine if partial outlet obstruction in rabbits results in the generation of nitrotyrosine (NT). Materials and methods: Sixteen rabbits were separated into four groups of four. The rabbits in groups 1 and 2 underwent sham operation while rabbits in groups 3 and 4 underwent partial outlet obstruction. The rabbits in groups 1 and 3 were evaluated after 1 week of obstruction and the rabbits in groups 2 and 4 were evaluated after 2 weeks of obstruction. A separate group of four controls were evaluated simultaneously with the sham and obstructed rabbits. Four rabbits from each group were evaluated after 1 and 2 weeks of obstruction. Four control rabbits were also evaluated. Isolated strips were evaluated for contractile responses and NT content of the mucosa and muscle were quantitated by Western blot analysis. Results: (1) The mucosa contains both 42 and 62 kD proteins exhibiting a strong nitrotyrosine signal; the muscle presents a signal only at 62 kD. (2) The sham operations had no effect on nitrotyrosine distribution or content. (3) The nitrotyrosine of both mucosal proteins and the muscle protein are increased in the 1 week obstructed bladder; whereas, only the 62 kD signal is increased in the two week obstructed bladder mucosa. (4) The contractile response to FS are reduced to a significantly greater degree than the responses to carbachol, KCl, or ATP. Conclusions: These studies clearly demonstrated that partial outlet obstruction in rabbits results in significant increases in nitrotyrosine within the bladder and may contribute to the contractile dysfunctions mediated by partial outlet obstruction. (Mol Cell Biochem 276: 143–148, 2005)
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References
Palmer RM, Ferrige AG, Moncada S: Nitric oxide release accounts for biological activity of endothelium-derived relaxing factor. Nature 327: 524–526, 1987
Arthur L, Burnett MD: Nitric oxide control of lower urinary tract functions: A review. Urology 45: 1071–1080, 1995
Andersson KE, Mattiasson A, Sjogren C: Electrically induced relaxation of the noradrenaline contracted isolated urethra from rabbit and man. J Urol 129: 210–214, 1983
Klarskov P, Gerstenberg T, Ramirez D, Hold T: Noncholinergic nerve mediated relaxation of trigone, bladder neck, and urethral smooth muscle in vitro. J Urol 129: 848–850, 1983
Persson K, Igawa Y, Mattiasson A, Andersson KE: Effects of inhibition of the l-arginine/nitric oxide pathway in the rat lower urinary tract in vivo and in vitro. Br J Pharmacol 107: 178–184, 1992
Chung BH, Choi SK, Chang KC: Effects of nitric oxide on detrusor relaxation. J Urol 155: 2090–2093, 1996
Kozlowski R, Siroky MB, Krane RJ, Azadzoi KM: Nitric oxide regulates rabbit bladder blood flow and microcirculation resistance (abstract). Neurourol Urodyn 18: 337, 1999
Lieb J, Kogan B, Das AK, Leggett RE, Schröder A, Levin RM: The effect of urine volume and nitric oxide on basal bladder blood flow: Response to catheterization and drainage. Neurourol Urodyn 20: 115–124, 2001
Schröder A, Lieb J, Kogan BA, Levin RM: Increased blood flow after catheterization and drainage in the chronically obstructed rabbit urinary bladder. Urology 58: 295–300, 2001
Gow AJ, Farkouh CR, Munson DA, Posencheg MA, Ischiropoulos H: Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol 287: L262–L268, 2004
Ferro TJ, Gertzberg N, Selden L, Neumann P, Johnson A: Endothelial barrier dysfunction and p42 oxidation induced by TNF-alpha are mediated by nitric oxide. Am J Physiol. 272: L979–L988, 1997
Lieb J, Chichester P, Kogan B, Das AK, Leggett RE, Schroeder A, Levin RM: Rabbit urinary bladder blood flow changes during the initial stage of partial outlet obstruction. J Urol 164: 1390–1397, 2000
Barry MJ, Meigs JB: The natural history of benign prostatic hyperplasia. In: H. Lepor (ed). Prostatic Diseases, W.B. Saunders Co., Philadelphia, 2000, pp 106–115
Levin RM, Brading AF, Mills IW, Longhurst PA: Experimental models of bladder obstruction. In: H. Lepor (ed). Prostatic Diseases. Philadelphia: W. B. Saunders Co., Philadelphia, 1999, pp 169–196
Schröder A, Chichester P, Kogan BA, Longhurst PA, Lieb J, Levin RM: Effect of chronic bladder outlet obstruction on the blood flow of the rabbit urinary bladder. J Urol 165: 640–646, 2001
Mumtaz FH, Khan MA, Thompson CS, Morgan RJ, Mikhailidis DP: Nitric oxide in lower urinary tract: physiological and pathological implication. BJU Int 85: 567–578, 2000
Saito M, Miyagawa I: Direct detection of nitric oxide in rat urinary bladder during ischemia reperfusion. J Urol 162: 1490–1495, 1999
Felsen D, Dardashti K, Ostad M, Lemer ML, Gross SS, Chen J, Vaughan ED Jr, Poppas DP: İnductible nitric oxide synthase promotes pathophysiological concequences of experimental bladder outlet obstruction. J Urol 169: 1569–1572, 2003
Yanagi K: Effects of nitric oxide on bladder outlet obstruction in rats. Yonago Acta Medica 45: 19–26, 2002
Sibley GN: Developments in our understanding of detrusor instability. Br J Urol 80(Suppl 1): 54–61, 1997
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Levin, R.M., Agartan, C.A., Leggett, R.E. et al. Effect of partial outlet obstruction on nitrotyrosine content and distribution within the rabbit bladder. Mol Cell Biochem 276, 143–148 (2005). https://doi.org/10.1007/s11010-005-4061-5
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DOI: https://doi.org/10.1007/s11010-005-4061-5