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Serine dehydratase expression decreases in rat livers injured by chronic thioacetamide ingestion

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Abstract

Serine dehydratase (SerDH) is a gluconeogenic enzyme involved in the catabolism of serine, which is regulated by the composition of their diet and their hormonal status in rats. This study examines how chronic injury caused to the liver of rats by the ingestion of thioacetamide (TAA) affects SerDH protein, mRNA levels, enzyme kinetics and its tissue location. After 97 days’ oral intake of TAA, the activity of SerDH at all substrate concentrations assayed was about 60% lower than in controls. No significant differences in Km values were found between the treated group and controls. Immunoblotting and immunohistochemistry revealed a significant reduction in the level of SerDH protein in the livers of the treated rats. SerDH was detected specifically in the periportal zone of the hepatic acinus and this location did not change in response to TAA treatment. The level of SerDH mRNA, quantified by reverse transcription and polymerase chain reaction, was significantly lower in treated rats than in the controls. The present findings suggest that the SerDH expression is rendered to be down regulatory during chronic liver injury induced by TAA. These results enhance our understanding about the biochemical mechanisms implied in the control and integration of serine catabolism during liver injury in rat. (Mol Cell Biochem 268: 33–43, 2005)

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Abbreviations

SerDH:

serine dehydratase

TAA:

thioacetamide

Km:

Michaelis constant

cAMP:

3′,5′-cyclic adenosine monophosphate

PLP:

pyridoxal 5′-phosphate

ASAT:

aspartate aminotransferase

ALAT:

alanine aminotransferase

AP:

alkaline phosphatase

γ-GT:

gamma-glutamyl transferase

rGST-7-7:

glutathione transferase isoenzyme 7-7

Vmax:

maximum velocity

SDS-PAGE:

polyacrylamide-gel electrophoresis with sodium dodecyl sulphate

TBS:

Tris buffer solution

PBS:

phosphate buffer solution

RT-PCR:

reverse transcription and polymerase chain reaction

dNTP:

deoxynucleotide triphosphate

AMV-RT:

avian-myeloblastosis virus reverse transcriptase

cDNA:

complementary DNA

Tm:

melting temperature

SDH3:

SerDH cDNA probe

S.E.M.:

standard error of the mean

bp:

base pair(s)

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Authors and Affiliations

  1. Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Campus Las Lagunillas, Jaén, Spain

    Inmaculada López-Flores, Juan B. Barroso, Raquel Valderrama, Esther Martínez-Lara & Juan Peragón

  2. Cell Biology Section, Department of Experimental Biology, University of Jaén, Jaén, Spain

    Francisco J. Esteban & M. Ángeles Peinado

  3. Genetic Section, Department of Experimental Biology, University of Jaén, Jaén, Spain

    Francisco Luque

  4. Department of Biochemistry, Toyama Medical and Pharmaceutical University Faculty of Medicine, Toyama, Japan

    Hirofumi Ogawa

  5. Department of Biochemistry and Molecular Biology, Centre for Biological Sciences, University of Granada, Granada, Spain

    José A. Lupiáñez

Authors
  1. Inmaculada López-Flores
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  2. Juan B. Barroso
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  3. Raquel Valderrama
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  4. Francisco J. Esteban
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  5. Esther Martínez-Lara
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  8. Hirofumi Ogawa
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  9. José A. Lupiáñez
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  10. Juan Peragón
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Correspondence to Juan Peragón.

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López-Flores, I., Barroso, J.B., Valderrama, R. et al. Serine dehydratase expression decreases in rat livers injured by chronic thioacetamide ingestion. Mol Cell Biochem 268, 33–43 (2005). https://doi.org/10.1007/s11010-005-2995-2

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  • DOI: https://doi.org/10.1007/s11010-005-2995-2

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