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Spatial Organization of the Chicken α-Globin Gene Domain in Cells of Different Origins

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Abstract

Hybridization with an oligonucleotide array was used to map the regions of DNA anchorage to the nuclear matrix. Matrix-associated DNA served as a hybridization probe. To obtain the oligonucleotide array, 60-mer oligonucleotides regularly distributed throughout the genome region of interest at 2-kb intervals were immobilized on a nylon filter. The organization of DNA into loop domains was studied in a 100-kb region of chicken chromosome 16, including the α -globin gene cluster. A 40-kb DNA loop, which was fixed to the nuclear matrix and harbored all α-globin genes, was observed in erythroid cells. One of its anchorage regions colocalized with matrix associated region (MAR) and an insulator found previously in the 5′ region of the chicken α-globin gene domain. The spatial (domain-loop) organization of the α-globin gene cluster in lymphoid cells proved to be strikingly different from that in erythroid cells.

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334, Russia

    E. S. Ioudinkova & S. V. Razin

  2. Institut Gustave Roussy, Villejuif, France

    A. V. Petrov & Y. S. Vassetzky

Authors
  1. E. S. Ioudinkova
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  2. A. V. Petrov
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  3. Y. S. Vassetzky
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  4. S. V. Razin
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__________

Translated from Molekulyarnaya Biologiya, Vol. 39, No. 6, 2005, pp. 971–977.

Original Russian Text Copyright © 2005 by Ioudinkova, Petrov, Vassetzky, Razin.

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Ioudinkova, E.S., Petrov, A.V., Vassetzky, Y.S. et al. Spatial Organization of the Chicken α-Globin Gene Domain in Cells of Different Origins. Mol Biol 39, 851–856 (2005). https://doi.org/10.1007/s11008-005-0105-x

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