Abstract
The allele and genotype frequency distributions of four STRs (the LPL, vWA, FES/FPS, and F13B loci) commonly used in forensic medicine were studied with a sample of 200 ethnic Russians from Siberia. Genetic and molecular diversity of the four STRs was characterized in comparison with the American Caucasoid population. The set of the four STRs showed a high power of discrimination (PD = 0.99975). Comparison of the genetic variation at the four loci revealed a considerable difference between the Russian and American Caucasoid populations, precluding the use of data on allele frequencies in American Caucasoids for forensic testing in Russia. The results can be used as a reference in Siberia.
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REFERENCES
Gill P., Ivanov P.L., Kimpton C., et al. 1994. Identification of the remains of the Romanov family by DNA analysis. Nature Genet. 6, 130–135.
Deka R., Shriver M.D., Yu L.M., et al. 1995b. Intra-and interpopulation diversity at short tandem repeat loci in diverse populations of the world. Electrophoresis. 16, 1659–1664.
Goldstein D.B., Ruiz Linares A., Cavalli-Sforza L.L., Feldman M.W. 1996b. Genetic absolute dating based on microsatellites and the origin of modern humans. Proc. Natl. Acad. Sci. USA. 92, 6723–6727.
Destro-Bisol G., Maviglia R., Caglia A., et al. 1999. Estimating European admixture in African Americans by using microsatellites and a microsatelllite haplotype (CD4/Alu). Hum. Genet. 104, 149–157.
Bosch E., Calafell F., Santos F.R., et al. 1999. Variation in short tandem repeats is deeply structured by genetic background in the human Y chromosome. Am. J. Hum. Genet. 65, 1623–1638.
Stepanov V.A. 2002. Etnogenomika naseleniya Severnoi Evrazii (Ethnogenomics of Northern Eurasia). Tomsk: Pechatnaya Manufaktura.
Jobling M.A., Gill P. 2004. Encoded evidence: DNA in forensic analysis. Nature Rev. Genet. 5, 739–751
Promega Corp. 2002. Genetic identity reference information. Population statistics. http://www.promega.com.
Huckenbeck W., Scheil H.-G. 2003. The distribution of the human DNA-PCR polymorphisms. http://www.uniduesseldorf.de.
Kornienko I.V., Zemskova E.Yu., Frolova S.A., Yakushev V.V., Ivanov P.L. 2002. Studies on the allele polymorphism of molecular-genetic individualizing systems based on tetranucleotide tandem repeats LPL, vWA, and TH01 in the Russian population. Sud.-Med. Ekspert. 45, 12–14.
Pushkarev V.P., Pakhmanina L.V., Novikov P.I., Ivanov P.L. 2004. Stusies on the allele diversity of microsatellite loci D16S539, F13B, FESFPS, TH01, and TPOX in Caicasoids from the Ural region (Russia) using capillary electrophoresis. Sud.-Med. Ekspert. 47, 23–28.
Guo S., Thomson E. 1992. Performing the exact test of Hardy-Weinberg proportion for multiple alleles. Biometrics. 48, 361–372.
Goldstein D.B., Ruiz Linares A., Cavalli-Sforza L.L., Feldman M.W. 1995. An evaluation of genetic distances for use with microsatellite loci. Genetics. 139, 463–471.
Zhivotovsky L.A. 1991. Populyatsionnaya biometriya (Population Biometry). Moscow: Nauka.
Krueger J., Fuhrmann W., Liechte K.H., Steffens S. 1968. Zur Verwendung des Polymorphismus der sauren Erythrozytenphosphatase bei der Vaterschaftsbegutachtung. Dtsch. Z. Gerichtl. Med. 12, 127–146.
Jones D.A. 1972. Blood samples: Probability of discrimination. J. Forensic Sci. Soc. 12, 355–359.
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Translated from Molekulyarnaya Biologiya, Vol. 39, No. 6, 2005, pp. 965–970.
Original Russian Text Copyright © 2005 by Shorokhova, Stepanov, Udovenko, Novoselov, Puzyrev.
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Shorokhova, D.A., Stepanov, V.A., Udovenko, Y.D. et al. Genetic Variation and Discriminating Power of Four DNA Microsatellites in the Russian Population. Mol Biol 39, 845–850 (2005). https://doi.org/10.1007/s11008-005-0104-y
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DOI: https://doi.org/10.1007/s11008-005-0104-y