Abstract
An original yeast system with two reporter genes oriented oppositely to each other was developed to study the regulatory functions of noncoding genomic sequences. A fragment of HERV-K LTR from region 22-19 of human chromosome 7p22 was tested for promoter activity. The LTR 22-19 fragment was shown to initiate transcription of a reporter regardless of its orientation, and to control both reporters simultaneously. The promoter activity was compared for the LTR 22-19 fragment and two potent promoters in Saccharomyces cerevisiae cells. The activity of the LTR 22-19 fragment accounted for about 0.34% of the activity of the inducible GAL1 promoter and for 0.25% of the activity of the constitutive TDH promoter.
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REFERENCES
Sugimoto J., Nobuo M., Kinjo Y., Takasu N., Oda T., Jinno Y. 2001. Transcriptionally Active HERV-K genes: Identification, isolation and chromosomal mapping. Genomics. 72, 137–144.
Domansky A.N., Kopantzev E.P., Snezhkov E.V., Lebedev Y.B., Leib-Mosch C., Sverdlov E.D. 2000. Solitary HERV-K LTRs possess bi-directional promoter activity and contain a negative regulatory element in the U5 region. FEBS Lett. 472, 191–195.
Sverdlov E.D. 2000. Retroviruses and primate evolution. BioEssays. 22, 161–171.
Hughes J.F., Coffin J.M. 2001. Evidence for genomic rearrangements mediated by human endogenous retroviruses during primate evolution. Nature Genet. 29, 487–489.
Hughes J.F., Coffin J.M. 2002. A novel endogenous retrovirus-related element in the human genome resembles a DNA transposon: Evidence for evolutionary link? Genomics. 80, 453–455.
Vinogradova T.V., Leppik L.P., Nikolaev L.G., Akopov S.B., Kleiman A.M., Senyuta N.B., Sverdlov E.D. 2001. Solitary human endogenous retroviruses-K LTRs retain activity in vivo, the mode of which is different in different cell types. Virology. 290, 83–90.
Kapitonov V.V., Jurka J. 1999. The long terminal repeat of an endogenous retrovirus induces alternative splicing and encodes an additional carboxy-terminal sequence in the human leptin receptor. J. Mol. Evol. 48, 248–251.
Vinogradova T., Volik S., Lebedev Y., Shevchenko Y., Lavrentyeva I., Khil P., Grzeschik K.H., Ashworth L.K., Sverdlov E. 1997. Positioning of 72 potentially full size LTRs of human endogenous retroviruses HERV-K on the human chromosome 19 map. Occurrences of the LTRs in human gene sites. Gene. 199, 255–264.
Sverdlov E.D. 1999. Retroviral regulators of gene expression in the human genome as possible factors of its evolution. Bioorg. Khim. 25, 821–827.
Goldenkova I.V. 2002. Reporter systems: Possibilities of studying different aspects of gene expression regulation. Usp. Sovrem. Biol. 122, 515–526.
Maniatis, T., Fritsch, E.F., Sambrook, J. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, N.Y.: Cold Spring Harbor Lab. Press.
Goldenkova I.V., Musiichuk K.A., Piruzyan E.S. 2002. A reporter system based on thermostability of lichenase from Clostridium tharminallum: Application to studies on gene expression in prokaryotic and eukaryotic cells. Mol. Biol. 36, 868–876.
Piruzian E.S., Goldenkova I.V., Musiychuk K.A., Kobets N.S., Arman I.P., Bobrysheva I.V., Chekhuta I.A., Glazkova D. 2002. A reporter system for prokaryotic and eukaryotic cells based on the thermostable lichenase from Clostridium thermocellum. Mol. Gen. Genomics. 266, 778–786.
Glazkova D.V., Efimenko I.G., Legchilina S.P., Boznhold D., Greshik K.-Ch., Arman I.P. 2000. TAR cloning of the human chromosome 7 short arm in yeast and search for terminal repeats. Genetika. 36, 622–629.
Green L.A., Tischler A. 1976. Establishment of a nonadrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA. 73, 2424–2428.
Long Q., Bengra C., Li C., Kutlar F., Tuan D. 1998. A long terminal repeat of the human endogenous retrovirus ERV-9 is located in the 5′ boundary area of the human beta-globin locus control region. Genomics. 54, 542–555.
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Translated from Molekulyarnaya Biologiya, Vol. 39, No. 2, 2005, pp. 207–213.
Original Russian Text Copyright © 2005 by Abdeeva, Komakhin, Musiychuk, Goldenkova.
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Abdeeva, I.A., Komakhin, R.A., Musiychuk, K.A. et al. Development of a yeast vector system to study the regulatory functions of eukaryotic noncoding elements. Mol Biol 39, 185–190 (2005). https://doi.org/10.1007/s11008-005-0027-7
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DOI: https://doi.org/10.1007/s11008-005-0027-7