Abstract
Objective This study measures the relative performance of three methods for diagnosing bacterial vaginosis (BV) during pregnancy and assesses the implications of measurement for clinical practice and surveillance.
Methods A sample (n = 1,780) of English or Spanish speaking women, with a singleton intrauterine pregnancy and receiving prenatal care at a consortium of public health centers in Philadelphia were consecutively enrolled. Gram stain, clinician’s diagnosis, and a commercial test were the three diagnostic methods used to assess BV. Sensitivity, specificity, and the positive and negative predictive values of clinical diagnosis and the commercial test were assessed using the gram stain/Nugent score as a gold standard.
Results The prevalence of BV, measured on the same population, differed considerably depending on the diagnostic test used. The measured prevalences were 55% (Gram stain), 28.5% (clinician’s diagnosis), and 12.6% (commercial test). The prevalence of BV (diagnosed by gram stain) was twice as high among African American women compared to White women. Only 69% BV-positive high-risk women were treated for BV.
Conclusions Inaccurate diagnosis of BV leads to missed cases. The identification of true cases is critical for assigning treatment and for assessing treatment effectiveness. Clinician’s routine diagnosis fell short of recommended procedures and performed poorly compared to gold standard in case ascertainment. This inability to ascertain cases may have an impact on our ability to prevent preterm birth.
Similar content being viewed by others
References
Ventura, S. J., Martin, J. A., Curtin, S. C., & Mathews, T. J. (1999). Births: final data for 1997. National Vital Statistics Reports, 47(18), 1–96.
MacDorman, M. F., & Atkinson, J. O. (1999). Infant mortality statistics from the 1997 period linked birth/infant death data set. National Vital Statistics Reports, 47(23), 1–23.
Wood, N. S., Marlow, N., Costeloe, K., Gibson, A. T., & Wilkenson, A. R. (2000). Neurologic and Developmental Disability after Extremely Preterm Birth, New England Journal of Medicine, 343(6), 378–384.
England L. Preterm Singleton Births, US. Ferre C, Martin J, & Hogan V, (Eds.) Morbidity and Mortality Weekly Report, 48(9), 185–189. 3-12-1999.
Centers for Disease Control and Prevention (CDC). 2000. State-specific changes in singleton preterm births among black and white women–United States, 1990 and 1997. Morbidity and Mortality Weekly Report, 49(37), 837–840.
Hillier, S. L., Nugent, R. P., Eschenbach, D. A., Krohn, M. A., Gibbs, R. S., & Martin, D. H. et al. (1995). Association between bacterial vaginosis and preterm delivery of a low- birth-weight infant. The vaginal infections and prematurity study group [see comments]. New England Journal of Medicine, 333(26), 1737–1742.
Meis, P. J., Goldenberg, R. L., Mercer, B. M., Iams, J. D., Moawad, A. H., & Miodovnik, M. et al. (1998). The preterm prediction study: risk factors for indicated preterm births. Maternal-fetal medicine units network of the national institute of child health and human development. American Journal of Obstetrics and Gynecology, 178(3), 562–567.
Kurki, T., Hallman, M., Zilliacus, R., Teramo, K., & Ylikorkala, O. (1992). Premature rupture of the membranes: effect of penicillin prophylaxis and long-term outcome of the children. American Journal of Perinatology, 9(1), 11–16.
Hay, P. E., Lamont, R. F., Taylor-Robinson, D., Morgan, D. J., Ison, C., & Pearson, J. (1994). Abnormal bacterial colonisation of the genital tract and subsequent preterm delivery and late miscarriage. BMJ, 308(6924), 295–298.
Gratacos, E., Figueras, F., Barranco, M., Vila, J., Cararach, V., & Alonso, P. L. et al. (1998). Spontaneous recovery of bacterial vaginosis during pregnancy is not associated with an improved perinatal outcome. Acta Obstetrica et Gynecologica Scandinavica, 77(1), 37–40.
Gravett, M. G., Nelson, H. P., DeRouen, T., Critchlow, C., Eschenbach, D. A., & Holmes, K. K. (1986). Independent associations of bacterial vaginosis and Chlamydia trachomatis infection with adverse pregnancy outcome. JAMA, 256(14), 1899–1903.
McGregor, J. A., French, J. I., Jones, W., Milligan, K., McKinney, P. J., & Patterson, E. et al. (1994). Bacterial vaginosis is associated with prematurity and vaginal fluid mucinase and sialidase: results of a controlled trial of topical clindamycin cream. American Journal of Obstetrics and Gynecology, 170(4), 1048–1059.
Eschenbach, D. A., Gravett, M. G., Chen, K. C., Hoyme, U. B., & Holmes, K. K. (1984). Bacterial vaginosis during pregnancy. An association with prematurity and postpartum complications. Scandinavian Journal of Urology and Nephrology Supplementum, 86, 213–222.
Taha TE, Hoover DR, Dallabetta GA, et al. Bacterial vaginosis and disturbances invaginal flora: associations with increased acquisition of HIV. AIDS, 12(13), 1699–1706.
Goldenberg, R. L., Klebanoff, M. A., Nugent, R., Krohn, M. A., Hillier, S., & Andrews, W. W. (1996). Bacterial colonization of the vagina during pregnancy in four ethnic groups. Vaginal Infections and Prematurity Study Group. American Journal of Obstetrics and Gynecology, 174(5), 1618–1621.
Culhane, J. F., Rauh, V., McCollum, K. F., Hogan, V. K., Agnew, K., & Wadhwa, P. D. (2001). Maternal stress is associated with bacterial vaginosis in human pregnancy. Maternal and Child Health Journal, 5(2), 127–134.
Fiscella, K. (1996). Racial disparities in preterm births. The role of urogenital infections [see comments]. Public Health Reports, 111(2), 104–113.
Taylor-Robinson, D. (1999). The future of bacterial vaginosis-related research. International Journal of Gynaecology and Obstetrics, 67(Suppl 1), S35–S38.
Nugent, R. P., Krohn, M. A., & Hillier, S. L. (1991). Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. Journal of Clinical Microbiology, 29(2), 297–301.
Amsel, R., Totten, P. A., Spiegel, C. A., Chen, K. C., Eschenbach, D., & Holmes, K. K. (1983). Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations. American Journal of Medicine, 74(1), 14–22.
Keane, F. E. A., Maw, R., Protchard, C., & Ison, C. A. (2005). Methods employed by genitourinary medicine clinics in the United Kingdom to diagnose bacterial vaginosis. Sexually Transmitted Infections, 81(2), 155–157.
Centers for Disease Control and Prevention. (1993) sexually transmitted diseases treatment guidelines. MMWR Morbidity Mortality Weekly Report, 42(RR-14), 1–102.
ACOG committee opinion. Bacterial vaginosis screening for prevention of preterm delivery. Number 198, February 1998. Committee on Obstetric Practice. American College of Obstetricians and Gynecologists. Int J Gynaecol Obstet 1998; 61(3), 311–312.
Koumans, E. H., Kendrick, J. S., & CDC Bacterial Vaginosis Working Group. (2001). Preventing adverse sequelae of bacterial vaginosis: public health program and research agenda. Sexually Transmitted Diseases, 28(5), 292–297.
Mardh, P. A., Tchoudomirova, K., Elshibly, S., & Hellberg, D. (1998). Symptoms and signs in single and mixed genital infections. International Journal of Gynaecology and Obstetrics, 63(2), 145–152.
West B., Morison, L., Vander Loeff, M., Gooding, E., Awasana, A., Demba, E. & Mayaud, P. (2003). Evaluation of a new rapid diagnostic kit (FemExam) for Bacterial vaginosis in patients with vaginal discharge syndrome in the Gambia. Sexually Transmitted Diseases, 30(6), 483–488.
Posner, S. F., Kerimova, J., Aliyeva, F., & Duerr, A. (2005). Strategies for diagnosis of bacterial vaginosis in a resource poor setting. International Journal of STD and AIDS, 16, 52–55.
Crowley, T., Berry, J., Horner, P. J., Gough, K. R., & Turner, A. (1998). Can a laboratory diagnosis of bacterial vaginosis be made from a transported high vaginal swab using anaerobic culture and microscopy of a wet preparation? [letter]. Sexually Transmitted Infections, 74(3), 228.
Donders, G. G., Vereecken, A., Dekeersmaecker, A., Van Bulck, B., & Spitz, B. (2000). Wet mount microscopy reflects functional vaginal lactobacillary flora better than Gram stain. Journal of Clinical Pathology, 53(4), 308–313.
Faro, S. (1996). Vaginitis: Diagnosis and management. International Journal of Fertility Menopausal Studies, 41(2), 115–123.
O’Dowd, T. C., West, R. R., Winterburn, P. J., & Hewlins, M. J. (1996). Evaluation of a rapid diagnostic test for bacterial vaginosis. British Journal of Obstetrics and Gynaecology, 103(4), 366–370.
Schmidt, H., & Hansen, J. G. (2000). Diagnosis of bacterial vaginosis by wet mount identification of bacterial morphotypes in vaginal fluid. International Journal of STD & AIDS, 11(3), 150–155.
Schwebke, J. R., Hillier, S. L., Sobel, J. D., McGregor, J. A., & Sweet, R. L. (1996). Validity of the vaginal gram stain for the diagnosis of bacterial vaginosis. Obstetrics and Gynecology, 88(4 Pt 1), 573–576.
Tam, M. T., Yungbluth, M., & Myles, T. (1998). Gram stain method shows better sensitivity than clinical criteria for detection of bacterial vaginosis in surveillance of pregnant, low- income women in a clinical setting [see comments]. Infectious Diseases in Obstetrics and Gynecology, 6(5), 204–208.
Thinkhamrop, J., Lumbiganon, P., Thongkrajai, P., Chongsomchai, C., & Pakarasang, M. (1999). Vaginal fluid pH as a screening test for vaginitis. International Journal of Gynaecology and Obstetrics, 66(2), 143–148.
Byrne, M. A., Turner, M. J., Griffiths, M., Taylor-Robinson, D., & Soutter, W. P. (1991). Evidence that patients presenting with dyskaryotic cervical smears should be screened for genital-tract infections other than human papillomavirus infection. European Journal of Obstetrics, Gynecology, and Reproductive Biology, 41(2), 129–133.
Carr, P. L., Felsenstein, D., & Friedman, R. H. (1998). Evaluation and management of vaginitis. Journal of General Internal Medicine, 13(5), 335–346.
Holzman, C., Leventhal, J. M., Qiu, H., & Jones, N. (2001). Wang J and the BV Study Group. Factors linked to bacterial vaginosis in non-pregnant women. American Journal of Public Health, 91(10), 1664–1670.
Author information
Authors and Affiliations
Corresponding author
Additional information
This work was supported by the ATPM/CDC Cooperative Agreement # TS 0286.
Rights and permissions
About this article
Cite this article
Hogan, V.K., Culhane, J.F., Hitti, J. et al. Relative performance of three methods for diagnosing bacterial vaginosis during pregnancy. Matern Child Health J 11, 532–539 (2007). https://doi.org/10.1007/s10995-007-0205-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10995-007-0205-4