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Inhibitory Effects of Antimicrobial Peptides from Lactobacillus casei HZ1 on Lipopolysaccharide-Induced RAW264.7 Macrophages Inflammation

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Abstract

Antimicrobial peptides (AMPs) have been found to be effective in neutralizing lipopolysaccharide (LPS) due to their cationic characteristics. LHH1 and LGH2 are two AMPs that we previously identified from the genome of Lactobacillus casei HZ1, which is originated from Chinese traditional fermented milk. In present study, the anti-inflammatory capacity of the two AMPs was investigated via a LPS-induced RAW264.7 macrophages inflammation model. Cell viability assay results showed that the AMPs showed a lower cytotoxicity on RAW264.7 cells at a concentration of lower than 32 μM. Compared to LGH2, LHH1 exhibited a good anti-inflammatory effect with a markedly declined release of nitric oxide (NO) from RAW264.7 cells. What’s more, LHH1 could effectively decrease the secretion of pro-inflammatory TNF-α, IL-6 and IL-1β. ITC data depicted that LHH1 could interact with LPS in an exothermic reaction. These data confirmed the efficacy of LHH1 from Lactobacillus casei HZ1 in exerting anti-inflammatory activity, which provided a novel AMP candidate in treating inflammation-related diseases.

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Data Availability

The data presented in this study are available on request from the corresponding author.

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Acknowledgements

We thank Natural Science Fund for Colleges and Universities in Jiangsu Province, China (21KJB350021) for financially support.

Funding

This work was financially supported by Natural Science Fund for Colleges and Universities in Jiangsu Province, China (21KJB350021).

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Authors and Affiliations

  1. Guangling College, Yangzhou University, Yangzhou, 225009, Jiangsu, People’s Republic of China

    Du-Xin Jin

  2. School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, People’s Republic of China

    Du-Xin Jin & Jun-Fang He

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  1. Du-Xin Jin
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  2. Jun-Fang He
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H-JF proposed the study; J-DX and H-JF performed the experiment and statistical analysis; J-DX drafted the manuscript.

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Correspondence to Du-Xin Jin.

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Jin, DX., He, JF. Inhibitory Effects of Antimicrobial Peptides from Lactobacillus casei HZ1 on Lipopolysaccharide-Induced RAW264.7 Macrophages Inflammation. Int J Pept Res Ther 29, 10 (2023). https://doi.org/10.1007/s10989-022-10484-0

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