Abstract
Gentamicin is a common aminoglycoside antibiotic used in clinical practice. However, its use is being increasingly restricted owing to its adverse effect on the inner ear. In this study, we tried to elucidate the mechanism behind the otoprotective effects exerted by a novel active peptide from Rapana venosa in zebrafish larvae. Our results showed that the combined application of gentamicin and this novel peptide helped the sensory hair cells reduce the uptake of gentamicin and subsequently restore the expression of nuclear factor erythroid-derived-2-like 2 (Nrf2), manganese superoxide dismutase (Mn-SOD), and copper/zinc superoxide dismutase (Cu/Zn-SOD). This, in turn, reduced excessive reactive oxygen species production and the incidence of apoptosis in sensory hair cells upon gentamicin administration. As a result, this peptide protected the sensory hair cells from gentamicin-induced loss. Our findings provide new insight into the application potential of an active peptide derived from marine food resources in the field of hearing protection.
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Acknowledgements
We would like to thank Editage (www.editage.cn) for English language editing.
Funding
This work was supported by the Shandong Provincial Natural Science Foundation for Fostering (Grant Number ZR2019PH011), the International Science and Technology Cooperation Program of Shandong Academy of Sciences (Grant Number 2019GHZD10), the National Key R&D Program of China (Grant Number 2018YFC1707300), and the Mount Taishan Scholar Program (Grant Number tspd20181211).
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All animal experiments complied with the ARRIVE guidelines and were carried out in accordance with the experimental protocol approved by the Ethics Committee of the Biology Institute at Qilu University of Technology.
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Gao, Y., Zhang, S., Wan, J. et al. A Novel Active Peptide from Rapana venosa Protects Against Gentamicin-Induced Sensory Hair Cell Loss in Zebrafish. Int J Pept Res Ther 27, 641–649 (2021). https://doi.org/10.1007/s10989-020-10114-7
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DOI: https://doi.org/10.1007/s10989-020-10114-7