Study on Interaction of Coomassie Brilliant Blue G-250 with Bovine Serum Albumin by Multispectroscopic

Yang, Mingjun; Shi, Dongxiang; Wang, Yonggang; Ebadi, Abdol Ghaffar; Toughani, Mohsen

doi:10.1007/s10989-020-10096-6

Published: 20 July 2020

Study on Interaction of Coomassie Brilliant Blue G-250 with Bovine Serum Albumin by Multispectroscopic

Mingjun Yang¹,
Dongxiang Shi¹,
Yonggang Wang¹,
Abdol Ghaffar Ebadi² &
Mohsen Toughani³

International Journal of Peptide Research and Therapeutics volume 27, pages 421–431 (2021)Cite this article

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Abstract

Aim of present study was to investigate the interaction of coomassie brilliant blue G-250 (CBBG-250) with bovine serum albumin (BSA) by the multispectroscopic methods. Fluorescence-data showed that the complex of BSA-CBBG-250 forming made the intrinsic fluorescence quenching of BSA by CBBG-250 interaction. BSA also could interact with CBBG-250 and the CBBG-BSA complexes formed in a molar ratio of 1:1. UV–Vis results displayed that the apparent binding (association) constant K_a of CBBG-250 with BSA was 5.03 × 10⁴ (298 K), 3.04 × 10⁴ (303 K), 2.84 × 10⁴ (308 K) and 1.99 × 10⁴ (313 K) L mol⁻¹ at different temperatures, respectively. The enthalpy change (△H) and entropy change (△S) were respectively calculated to be − 45.32 kJ mol⁻¹ and − 139.18 J mol⁻¹ K⁻¹, indicating that the hydrogen bonds and Van der Waals forces played dominant roles in the interaction. The results showed that the diphenylamine structure and amino acid residues in the Coomassie Brilliant Blue G-250 had a strong Van der Waals force. The phenyl sulphonic acid group undergoes electrostatic interactions and hydrogen bond interactions with basic amino acids; the compound Coomassie Brilliant Blue G-250 can form a stable complex with BSA.

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Acknowledgement

We gratefully acknowledge the support from University innovation fund of Gansu Province (No. 2020).

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Authors and Affiliations

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China
Mingjun Yang, Dongxiang Shi & Yonggang Wang
Department of Agriculture, Jouybar Branch, Islamic Azad University, Jouybar, Iran
Abdol Ghaffar Ebadi
Department of Fishery, Babol Branch, Islamic Azad University, Babol, Iran
Mohsen Toughani

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Mingjun Yang
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Dongxiang Shi
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Yonggang Wang
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Abdol Ghaffar Ebadi
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Mohsen Toughani
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Correspondence to Yonggang Wang.

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Yang, M., Shi, D., Wang, Y. et al. Study on Interaction of Coomassie Brilliant Blue G-250 with Bovine Serum Albumin by Multispectroscopic. Int J Pept Res Ther 27, 421–431 (2021). https://doi.org/10.1007/s10989-020-10096-6

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Accepted: 02 July 2020
Published: 20 July 2020
Issue Date: March 2021
DOI: https://doi.org/10.1007/s10989-020-10096-6

Keywords

Bovine serum albumin
Coomassie brilliant blue G-250
Interaction
Spectrum