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Expression and Purification of Membrane Proteins in Different Hosts

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Abstract

Membrane proteins play important functions, such as cellular communication and transferring materials in the cell. Many membrane proteins are involved in human diseases. However, little information is available on their structure, stability and folding. To study their structure, membrane proteins should primarily be produces in large amounts. However, production of these proteins is limited by various technical issues. Developing novel strategies to circumvent these issues seems to be highly important to proceed in membrane protein science. In this review, we have summarized the recent findings which can promote membrane protein expression and purification.

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Authors and Affiliations

  1. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Fatemeh Abarghooi Kahaki

  2. Department of Biology, East Tehran Branch Faculty of Science, Islamic Azad University, Tehran, Iran

    Sakineh Monzavi

  3. Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

    Hadi Bamehr

  4. Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Eshagh Bandani

  5. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Zahra Payandeh

  6. Applied Microbiology Research Center, Systems Biology, and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Abolfazl Jahangiri

  7. Department of Biology Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran

    Saeed Khalili

Authors
  1. Fatemeh Abarghooi Kahaki
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  2. Sakineh Monzavi
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  3. Hadi Bamehr
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  4. Eshagh Bandani
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  5. Zahra Payandeh
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  6. Abolfazl Jahangiri
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  7. Saeed Khalili
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Correspondence to Zahra Payandeh or Saeed Khalili.

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Abarghooi Kahaki, F., Monzavi, S., Bamehr, H. et al. Expression and Purification of Membrane Proteins in Different Hosts. Int J Pept Res Ther 26, 2077–2087 (2020). https://doi.org/10.1007/s10989-019-10009-2

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